MicroRNA-126对人肝细胞糖代谢的影响  

Effects of MicroRNA-126 on glucose metabolism in human liver cells

作　　者：胡志为[1] 何亚非[1] 刘艳霞[1] 姜榴茗[1] 彭家欣[1] 栗夏莲[1] 

机构地区：[1]郑州大学第一附属医院内分泌科,450052

出　　处：《中华糖尿病杂志》2015年第10期634-638,共5页CHINESE JOURNAL OF DIABETES MELLITUS

基　　金：河南省卫生厅省部共建项目（201201015）

摘　　要：目的探讨MicroRNA-126对人肝细胞糖代谢功能影响的机制。方法以来源于人肝脏的永生非肿瘤细胞系——张氏肝细胞为研究对象，分为6组，分别转染MicroRNA-126类似物与MicroRNA-126抑制物及其相应的阴性对照序列，并设正常对照组和脂质体Lipofectamine2000组。采用实时定量聚合酶链反应（RT-PCR）检测转染后各组细胞中MicroRNA-126的相对表达量，用Westernblotting法检测各组细胞中胰岛素受体底物1（IRS-1）、磷脂酰肌醇激酶p85p（PIK3R2）、蛋白激酶B2（Akt-2）、磷酸化Akt-2（P-Akt-2）的蛋白表达水平。采用单因素方差分析及t检验进行数据分析。结果RT．PCR检测结果显示：MicroRNA-126类似物转染组中MicroRNA-126的相对表达量明显高于正常对照组（799．70±66．46比1．00±0．00，t=20．82，P〈0．05）。MicroRNA-126类似物转染组细胞中IRS-1、PIK3R2的蛋白表达水平较正常对照组明显降低（分别为0．41±0．04比1．09±0．02、0．41±0．03比0．89±0．01，t=-25．53、-26．63，均P〈0．05）。各组问Akt-2的相对表达量差异无统计学意义（F=0．35，P〉0．05）。MicroRNA-126类似物转染组Akt-2的磷酸化水平（P-Akt-2／Akt-2）较正常对照组明显降低（0．53±0．07比0．88±0．09，t=-5．298，P〈0．05）。结论MicroRNA-126通过抑制IRS-1／PIK3R2／Akt-2胰岛素信号传导通路影响人肝细胞的糖代谢。Objective To investigate the effects of MicroRNA-126 on glucose metabolism in the human liver cells. Methods The immortal liver-derived cell line Chang liver cell was taken as the object. Chang liver cells were transfected with MicroRNA-126 mimic, MicroRNA-126 inhibitor and relative negative control respectively, meanwhile the normal control group and Lipofectamine 2000 group were set too. The expression level of MieroRNA-126 was detected by reverse transcription-polymerase chain reaction （RT-PCR）, and the protein expression of insulin receptor substrate-I（IRS-1）, phosphatidylinositol 3-kinase p8513（PIK3R2）, protein kinase B-2 （Akt-2） and phosphorylated Akt-2 （P-Akt-2） were detected by using Western blotting. Data were analyzed with one-way ANOVA or t test. Results The expression of MicroRNA-126 was significantly increased in MicroRNA-126 mimic group compared with that in normal control group （799.70±66.46 vs 1.00±0.00, t=20.82, P〈0.05）. Compared with the normal control group, the protein expression of IRS-1, PIK3R2 decreased in MicroRNA-126 mimic group（0.41±0.04 vs 1.09±0.02, 0.41±0.03 vs 0.89±0.01, t=-25.53,-26.63, both P〈0.05）.The differences in the expression of Akt-2 among the 6 groups was not statistically significant（F=0.35, P〉0.05）. Compared with that in the normal control group, the P-Akt-2/Akt-2 decreased in MieroRNA-126 mimic group （0.53±0.07 vs 0.88±0.09, t=-5.298, P〈 0.05）. Conclusion MieroRNA-126 may affect glucose metabolism in human liver cells by inhibiting IRS-1/ PIK3R2/Akt-2 signaling pathway.

关 键 词：MicroRNA-126 人肝细胞 糖代谢 

分 类 号：R587.1[医药卫生—内分泌]