The complete mitogenome of Lamproptera curia （Lepidoptera： Papilionidae） and phylogenetic analyses of Lepidoptera  

作　　者：Xin-Min Qin Qing-Xin Guan Hui-Min Li Yu Zhang Yu-Ji Liu Dan-Ni Guo 

机构地区：[1]Guangxi Key Laboratory of Rare and Endangered Animal Ecology, College of Life Science, Guangxi Normal University, Guilin 541004,China

出　　处：《Frontiers in Biology》2015年第5期458-472,共15页生物学前沿（英文版）

摘　　要：The complete mitochondrial genome sequence of Lamproptera curia was determined in the present study. Our findings showed that the mtDNA of L. curia had a typical organization of insect mitochrondrial DNA - being 15277 base pairs in length, it contained 13 protein-coding genes （PCGs）, 2 rRNA genes, 22 tRNA genes, and a control region （CR）. The newly determined sequence was used for phylogenetic analyses, together with those of 45 species of Lepidoptera published elsewhere, including sequences of three species of Diptera as outgroups. The phylogenetic trees were constructed using the concatenated amino acid and nucleotide sequences of the 13 protein-coding genes （PCGs） based on the maximum likelihood （ML） and Bayesian inference （BI） methods. Both BI and ML trees revealed a similar topology structure： （（（（（Bombycoidea ＋ Geometroidea） ＋ Noctuoide） ＋ Pyraloidea） ＋ （Papilionoidea ＋ Hesperioidea）） ＋ Tortricoidea）. Furthermore, the phylogenetic analyses demonstrated that each of the 16 families belonged to a monophyletic group respectively. The results of molecular phylogeny from the present study were congruent with traditional classification based on morphology but failed to demonstrate the monophyly of Hesperiidae.The complete mitochondrial genome sequence of Lamproptera curia was determined in the present study. Our findings showed that the mtDNA of L. curia had a typical organization of insect mitochrondrial DNA - being 15277 base pairs in length, it contained 13 protein-coding genes （PCGs）, 2 rRNA genes, 22 tRNA genes, and a control region （CR）. The newly determined sequence was used for phylogenetic analyses, together with those of 45 species of Lepidoptera published elsewhere, including sequences of three species of Diptera as outgroups. The phylogenetic trees were constructed using the concatenated amino acid and nucleotide sequences of the 13 protein-coding genes （PCGs） based on the maximum likelihood （ML） and Bayesian inference （BI） methods. Both BI and ML trees revealed a similar topology structure： （（（（（Bombycoidea ＋ Geometroidea） ＋ Noctuoide） ＋ Pyraloidea） ＋ （Papilionoidea ＋ Hesperioidea）） ＋ Tortricoidea）. Furthermore, the phylogenetic analyses demonstrated that each of the 16 families belonged to a monophyletic group respectively. The results of molecular phylogeny from the present study were congruent with traditional classification based on morphology but failed to demonstrate the monophyly of Hesperiidae.

关 键 词：Lamproptera curia PHYLOGENY mitochondrial genome 

分 类 号：Q969.42[生物学—昆虫学] S852.659.5[农业科学—基础兽医学]