补气活血通络颗粒对博莱霉素致小鼠肺纤维化研究  

Experimental Study of Buqi Huoxue Tongluo Granule to Bleomycin Treatment Induced Pulmonary Fibrosis in Mice

作　　者：刘建博[1] 张强[1] 李宇杰[1] 刘海枝[1] 蔡俊翔[1] 

机构地区：[1]广州中医药大学第一附属医院,广东广州510405

出　　处：《辽宁中医药大学学报》2015年第11期5-7,共3页Journal of Liaoning University of Traditional Chinese Medicine

基　　金：广东省自然科学基金资助项目(S2012010010574)

摘　　要：目的:研究中药补气活血通络颗粒对博来霉素致肺纤维化的防治作用。方法:60只小鼠随机分5组:对照组、空白组、中药高剂量组、低剂量组及西药组。灌胃7 d后提取肺组织,ELISA法测定HYP含量,ELISA法测定肺组织匀浆TNF-α含量,予以病理形态观察。结果:各药物干预组与空白组比较,小鼠肺系数明显下降(P<0.05),肺泡炎、纤维化程度均有改善(P<0.05),肺HYP含量下降(P<0.05),肺组织匀浆TNF-α含量下降(P<0.05)。结论:补气活血通络颗粒在某种程度上减轻BLM诱导的肺泡炎及肺纤维化程度,同时可能对于肺HYP及TNF-α含量亦有明显的抑制作用,提示补气活血通络颗粒对肺纤维化有一定预防及治疗作用。Objective：To assess preventive and therapeutic effect of Chinese herbs of Buqi Huoxue Tongluo Granule on bleomycin induced monary fibrosis. Methods：60 mouse were randomly divided into five groups,control group,blank group,high dose of Chinese medicine group,low dose group and western medicine group.Extraction of lung tissue after intragastric administration of 7 days,determination of HYP content with ELISA method,determination of the content of the lung tissue homogenate TNF-α by ELISA method,pathological observation. Results：Each medicine intervention group compared with the blank group,lung index of mice was obviously decreased（P〈0.05）,alveolitis,fibrosis degree were significantly improved（P〈0.05）,pulmonary HYP content were significantly decreased（P〈0.05）. Conclusion：Buqi Huoxue Tongluo Granule decreased BLM induced alveolitis and pulmonary fibrosis in certain extent.At the same time,it could also inhibit the content of HYP and TNF-α.

关 键 词：补气活血通络颗粒 肺纤维化小鼠 博来霉素 

分 类 号：R563[医药卫生—呼吸系统]