丹藤颗粒质量标准研究  

Research on Quality Standard of Danteng Granule

作　　者：王爱武[1] 李美艳[1,2] 刘丽清[1,2] 刘帅[1] 

机构地区：[1]山东大学附属省立医院,山东济南250021 [2]山东中医药大学,山东济南250355

出　　处：《辽宁中医药大学学报》2015年第11期8-11,共4页Journal of Liaoning University of Traditional Chinese Medicine

摘　　要：目的：建立丹藤颗粒的质量标准。方法：采用薄层色谱法对制剂中丹参、首乌藤、葛根进行定性鉴别,采用高效液相色谱法测定丹酚酸B含量,色谱柱为Agilent Eclipse XDB-C18柱（250 mm×4.6 mm,5μm）,流动相为A-乙腈,B-乙腈/0.3%甲酸（5/95）梯度洗脱,流速为1.0 m L·min-1,测定波长为280 nm,柱温为20℃。结果：丹参、首乌藤、葛根的薄层鉴别斑点清晰,专属性强;丹酚酸B在60~540μg/m L范围内呈良好的线性关系,=0.9994,平r均回收率为97.93%,RSD为1.02%。结论：该方法简便、灵敏、准确、重复性好,可作为丹藤颗粒的质量控制标准。Objective：To set up the quality standard for Danteng Granule. Methods：TLC was applied to the qualitative identification of Salviae miltiorrhizae,Polygoni Caulis and gegen. The content of salvianolic acid B was determined by HPLC,The column was Agi Lent Ec Lipse XDB-C18（250 mm ×4.6 mm,5 μm）with the mobile phase composed of acetonitrile-acetonitrile/0.3% formic acid（5/95）gradient elution. The flow rate was 1.0 m L·min-1,the detection wavelength was 280 nm,the column temperature was 20 ℃. Results：The spots of Salviae miltiorrhizae,Polygoni Caulis and gegen TLC plates were clear and specific.A good linearity of salvianolic acid B was in the range of 60~540 μg/m L（r=0.9994）. The average recovery of salvianolic acid B was 97.93%,and RSD was 1.02%. Conclusion：The methods are simple,sensitive,accurate and with good reproducibility. It can be used for the quality control of Danteng Granule.

关 键 词：丹藤颗粒 薄层色谱法 高效液相色谱法 丹酚酸B 质量标准 

分 类 号：R284[医药卫生—中药学]