飞龙掌血总生物碱提取工艺研究  

Study on the Optimization of Extraction Process of Total Alkaloids in Toddalia asiatica Lam

作　　者：刘思艺[1] 谷悦[1] 赵俐婷[1] 叶嘉敏 赵钟祥[1] 张蕾[1] 卢丽萍[1] 

机构地区：[1]广州中医药大学中药学院,广东广州510006

出　　处：《辽宁中医药大学学报》2015年第11期65-67,共3页Journal of Liaoning University of Traditional Chinese Medicine

基　　金：广东省省级大学生创新实验项目(1057213014)

摘　　要：目的:优选飞龙掌血总生物碱的提取工艺。方法:以酸性染料比色法测定的总生物碱含量为指标,采用单因素和正交试验法,考察液料比、乙醇体积分数、提取温度对提取工艺的影响,确定最佳提取工艺。结果:各因素对乙醇回流提取工艺的影响顺序为提取温度、液料比、乙醇体积分数,最佳提取工艺为用25倍量75%乙醇,75℃回流提取40 min。结论:优选的提取工艺稳定可行,提取率高,为飞龙掌血资源的充分利用提供实验依据。Objective：To optimize the extraction process of total alkaloids in Toddalia asiatica. Methods：With extraction amount of total alkaloids determined by acid dye colorimetry as index,effect of solid-liquid ratio,ethanol concentration,extraction temperature,the best process was screened by single factor and orthogonal experimental design. Results：The influencing order of each factor on ethanol reflux extraction process was extraction temperature,solid-liquid ratio,ethanol concentration. The optimum extraction process is as following：extracted 40 min with 25 times the amount of 75% ethanol at 75 ℃. Conclusion：Optimized technology is stable and feasible with high extraction rate,and it provides the experimental basis for full use of Toddalia asiatica.

关 键 词：飞龙掌血 总生物碱 正交试验 提取工艺 

分 类 号：R284.2[医药卫生—中药学]