经硬式鼻腔镜气管内滴注化学制剂建立AECOPD动物模型  

Establish Animal Models of AECOPD by Drop Chemicals in through Rigid Nasal Mirror

作　　者：封继宏[1] 祁海燕[2] 郑兆晔[1] 刘伟[1] 苏景深[1] 关鹏[1] 杨爽[2] 李云辉[2] 李美凤[1] 孙增涛[1] 

机构地区：[1]天津中医药大学第二附属医院,天津300150 [2]天津中医药大学,天津300193

出　　处：《辽宁中医药大学学报》2015年第11期68-70,共3页Journal of Liaoning University of Traditional Chinese Medicine

基　　金：国家自然科学基金资助项目(81202796)

摘　　要：目的:寻求一种在损伤最小的情况下建立慢性阻塞性肺病急性加重期(AECOPD)大鼠模型的新方法。方法:将40只大鼠分为正常对照组、模型组,乙醚麻醉后,在硬式鼻腔镜引导及松下摄录监视系统下,使用自制鼠麻醉喉镜,气管内隔周定期注入弹性蛋白酶,并最后滴入细菌,从而建立AECOPD大鼠模型。结果:模型组大鼠精神萎靡,动则气喘,呼吸深大,气道分泌物增多;肺组织体积增大、出现瘀血,病理结果显示肺泡壁变薄变大,肺泡腔融合增大,部分破裂融合形成肺大泡,肺泡数目明显减少,肺泡周围可见炎细胞浸润,改变与人类COPD改变相符合。模型组大鼠肺功能FEV0.3、FEV0.3/FVC、FVC较正常对照组显著下降。结论:通过运用硬式鼻腔镜、松下摄录监视系统及自制鼠麻醉喉镜,气管内滴入弹性蛋白酶,并结合细菌攻击可以成功建立AECOPD大鼠模型。Objective：To find a new way for the establishment of chronic obstructive pulmonary disease with acute exacerbation（AECOPD） in rats with minimal damage. Methods：40 rats were divided into normal control group and medel group. After anesthetized,drop elastase with the help of rigid nasal mirror and homemade laryngoscope every other week on a regular basis,finally drop bacterial to establish rats models of AECOPD. Results：The model group：listlessness,out of breath after moving,deep breath,increased airway secretions,lung volume increases,congestion. Pathology showed：alveolar wall become thinner and larger,alveolar fusion increases,broken parts form bulla,the number of alveolar reduced,inflammatory cell infiltration were seen around. It is consistent with human COPD.Lung function of FEV0.3,FEV0.3/FVC and FVC was significantly decreased in model group,compared with the control group. Conclusion：Animal model of AECOPD can be successfully established by drop elastase and Bacterial,with the rigid nasal mirror and homemade laryngoscope.

关 键 词：慢性阻塞性肺疾病急性加重 动物模型 硬式鼻腔镜 弹性蛋白酶 细菌 

分 类 号：R563[医药卫生—呼吸系统]