响应面法优选钩藤的乙醇温浸工艺  

Optimization of Warm Ethanol Immersion Technology of Uncaria Using Response Surface Methodology

作　　者：张群[1] 王燕[1] 尹雷[1] 孙志强[1] 代龙[1] 

机构地区：[1]山东中医药大学药学院,山东济南250355

出　　处：《辽宁中医杂志》2015年第11期2154-2156,I0003,共4页Liaoning Journal of Traditional Chinese Medicine

基　　金：国家"重大新药创制"科技重大专项(2012ZX09102201-004)

摘　　要：目的:用响应面法优选钩藤乙醇温浸工艺的最佳条件。方法:以异钩藤碱的提取率为响应值,乙醇浓度、次数、温度、时间为自变量,在单因素实验的基础上采用4因素3水平响应面法进行试验设计,确定温浸的最佳条件。结果:温浸的最佳条件为:乙醇浓度84.82%,时间1.5 h,温度80℃,提取2.83次,异钩藤碱提取率的预测值为81.97%,实际值为79.93%。结论:响应面法对钩藤的温浸条件优化合理可行,为提高异钩藤碱的提取率提供了理论依据。Objective： To optimize the conditions for Uncaria warm ethanol immersion by the response surface method. Methods： By taking the isorhynchophylline extraction rate as the response value and ethanol concentration,frequency,temperature and time as the independent variable and based on single factor experiment,4 factors and 3 levels of response surface method was used to determine the optimal conditions of warm ethanol immersion. Results： The best conditions of were ethanol concentration84. 82%,time 1. 5 hours,temperature 80 ℃,2. 83 times of extraction. The prediction of isorhynchophylline extraction rate was81. 97% and the actual value was 79. 93%. Conclusion： Of Uncaria warm ethanol immersion,the response surface method is reasonable and feasible for the conditions optimization,providing the theory basis for improving the extraction rate of isorhynchophylline.

关 键 词：钩藤 温浸 异钩藤碱 响应面法 

分 类 号：R284[医药卫生—中药学]