月光银桦组织培养技术  

Technique on tissue culture of Grevillea moonlight

作　　者：焦金凤[1] 张晓明[1] 邓小梅[1] 奚如春[1] 

机构地区：[1]华南农业大学广东省森林植物种质创新与利用重点实验室,广州510642

出　　处：《林业科技开发》2015年第6期73-76,共4页China Forestry Science and Technology

基　　金：国家林业公益性行业科研专项(201404116)

摘　　要：对月光银桦组织培养技术进行了研究,筛选出了适合的诱导培养基为1/2 MS+6-BA 1.0 mg/L+NAA0.1 mg/L+GA31.0 mg/L,诱导率达71%;较适合的增殖培养基为1/2 MS+6-BA 0.1 mg/L+NAA 0.01 mg/L,增殖系数达3.25;较适合的生根培养基为1/2MS+IBA 0.5 mg/L+NAA 0.5mg/L,生根率达92.3%。移栽基质为泥炭土+珍珠岩(V泥炭土:V珍珠岩=3∶1),移栽成活率可达85%以上。The tissue culture techniques for G. moonlight were studied. The results showed that the suitable induction medium was 1/2 MS ＋6-BA 1.0 mg/L ＋ NAA 0.1 mg/L ＋ GA3 1.0 mg/L,induction rate was 71%. The suitable proliferation medium was 1/2 MS ＋6-BA 0.1 mg/L ＋0.01 mg/L NAA,the multiplication coefficient was 3.25 roughly. The suitable rooting medium was 1/2 MS ＋ IBA 0.5 mg/L ＋0.5 mg/L NAA,rooting rate was 92.3%. The transplanting substrate was peat and perlite （ Vpeat ： Vperlite = 3： 1 ）, transplanting survival rate conld reach more than 85 %.

关 键 词：月光银桦 组织培养 增殖培养 

分 类 号：S722.37[农业科学—林木遗传育种]