小鼠骨髓间充质干细胞SCA-1^+/CD45^+/CD31^+亚群抗凋亡能力的研究  

Preliminary study of the anti-apoptotic ability of mouse bone marrow mesenchymal stem cell subsets SCA-1^+/CD45^+/CD31^+ during repair of myocardial infarction

作　　者：贺继刚[1] 李洪荣[1] 桂龙升[1] 李永武[1] 严丹[1] 王平[1] 

机构地区：[1]云南省第一人民医院心脏大血管外科,昆明650032

出　　处：《临床心血管病杂志》2015年第11期1223-1226,共4页Journal of Clinical Cardiology

基　　金：国家自然科学基金项目(No:81460073);云南省科技厅-昆明医科大学应用基础研究联合专项(No:2014FB089)

摘　　要：目的:探讨小鼠骨髓间充质干细胞(mBMSCs)各亚群在心肌修复中抗凋亡能力。方法:以小鼠心肌干细胞表面分化抗原检测mBMSCs后以CD45、CD31为标准分选得到小鼠骨髓间充质干细胞4个亚群。体外将4个亚群采用无血清低氧(5%)培养,流式细胞仪检测各组的凋亡率。分别将细胞注入小鼠心梗模型体内,注射后48h完成心功能测定。结果:SCA-1+/CD45+/CD31+组抗凋亡能力最强,心脏彩超提示心功能恢复亦优于其他各组。结论:小鼠骨髓间充质干细胞SCA-1+/CD45+/CD31+组抗凋亡能力明显优于其他组,治疗后心功能恢复亦优于其他各组。Objective：To explore and compare the anti-apoptotic abilities of all subsets of mice bone marrow mesenchymal stem cells（mBMSCs）during myocardial repair.Method：Four subsets of mBMSCs were obtained by separating them with CD45 and CD31 as the standards after surface differentiation antigen detection of mBMSCs.After in vitro cell culture in a serum-free medium with low oxygen content（5%）,the apoptosis rates of the four subsets were measured via flow cytometry.Heart function was detected within 48 hafter infusing the cells into the mice 24 hafter MI.Result：The group treated with SCA-1＋/CD45＋/CD31＋showed the strongest anti-apoptotic ability with enhanced recovery of cardiac function as revealed via echocardiography.Conclusion：The group of mBMSCs treated with SCA-1＋/CD45＋/CD31＋exhibited markedly superior anti-apoptotic ability and improved recovery of cardiac function.

关 键 词：小鼠骨髓间充质干细胞 亚群 心肌梗死 抗凋亡 

分 类 号：R542.2[医药卫生—心血管疾病]