人参蛋白对过氧化氢致神经元损伤的保护作用  

Neuroprotective effects of ginseng protein on neurons against damages induced by hydrogen peroxide

作　　者：李红艳[1] 代永霞[2] 张江华[3] 孔亮[1] 康廷国[1] 杨静娴[1] 

机构地区：[1]辽宁中医药大学药学院,辽宁大连116600 [2]郑州澍青医学高等专科学校基础医学部,河南郑州450064 [3]大连工业大学轻工与化学工程学院,辽宁大连116034

出　　处：《中国医院药学杂志》2015年第21期1906-1910,共5页Chinese Journal of Hospital Pharmacy

基　　金：中国博士后科学基金项目(编号:2013M530945)

摘　　要：目的:采用H2O2诱导小鼠神经元损伤,研究人参蛋白(GP)对神经元的保护作用。方法:从新生乳鼠大脑皮层中分离纯化神经元,通过倒置荧光显微镜观察细胞形态,β-半乳糖苷酶试剂盒检测细胞衰老,MTT法检测细胞存活,Hoechst 33342/PI双染色法检测细胞凋亡与坏死,荧光定量RT-PCR实验检测凋亡基因Bcl-2、Bax mRNA表达。结果:H2O2致细胞出现轴突缩短或消失,胞体变圆缩小,大小不等,核固缩,核染色质聚集等形态学变化,细胞存活率降低,Hoechst33342及β-半乳糖苷酶染色阳性;人参蛋白可改善细胞形态,增加细胞存活率,减少细胞凋亡与坏死,降低细胞衰老率。结论:人参蛋白对H2O2致神经元损伤具有保护作用,其机制与促进Bcl-2 mRNA表达,抑制Bax mRNA表达有关。OBJECTIVE To investigate neuroprotective effects of ginseng protein（ GP） on mice neuron damages induced by hydrogen peroxide（ H2O2）. METHODS Primary neurons,isolated and purified from cerebral cortex of suckling mouse,were observed for cell morphology by using inverted fluorescence microscope. Cell senescence was detected by β-galactosidase assay kit,cell survival by MTT colorimetry,apoptosis and necrosis of cells by Hoechst 33342 / PI double staining method,and Bcl-2 and Bax mRNA expression by fluorescent quantitative RT-PCR. RESULTS After treatment of H2O2,axons shortened or disappeared,cell bodies became round,with reduced and unequal size. Karyopyknosis and aggregation of nuclear chromatin happened,cell survival rate decreased,and Hoechst 33342 staining and β-galactosidase staining showed positive results. Besides,ginseng protein could improve cell morphologies,increase cell survival rate,decrease cell apoptosis and necrosis,and reduce rate of cell senescence. CONCLUSION Ginseng protein has neuroprotective effects on neurons against damages induced by H2O2,which may be associated with induction of a pro-apoptotic gene Bcl-2 and inhibition of anti-apoptotic gene Bax mRNA expression in neurons.

关 键 词：人参蛋白 H2O2 细胞衰老 存活 凋亡 坏死 

分 类 号：R927.2[医药卫生—药学]