贝伐单抗对人脐静脉内皮细胞纤维化相关炎症因子表达的影响  

Effects of bevacizumab on expression of fibrosis-related inflammatory mediators in human umbilical vein endothelial cells

作　　者：储三军[1] 王敏[2] 徐海峰[2] 

机构地区：[1]青岛大学医学院,山东省眼科研究所,山东省青岛市266071 [2]山东省眼科研究所,青岛眼科医院,山东省青岛市266071

出　　处：《眼科新进展》2015年第11期1025-1028,共4页Recent Advances in Ophthalmology

基　　金：山东省自然科学基金资助(编号:ZR2014HM029);青岛市科技计划项目(编号:13-1-3-69-nsh)~~

摘　　要：目的观察贝伐单抗对人脐静脉内皮细胞(human umbilical vein endothelial cells,HUVEC)纤维化相关炎症因子表达的影响,以探讨贝伐单抗治疗后新生血管纤维化的可能机制。方法分别在正常氧含量及缺氧条件下培养HUVEC,培养液中加入终浓度为0.25g·L-1贝伐单抗,根据处理时间的不同分为0 h组(对照组)、6 h组、12 h组、24 h组、48 h组。分别用RT-PCR及ELISA方法检测各组纤维化相关炎症因子白细胞介素(interleukin,IL)-1β、IL-6、IL-8以及肿瘤坏死因子-α(tumor necrosis factor-alpha,TNF-α)mRNA和蛋白的表达。结果正常氧含量条件下,与对照组相比,IL-6、IL-8的mRNA和蛋白表达在各实验组均明显增加,而IL-1β、TNF-αmRNA和蛋白的表达仅在12 h、24 h、48 h组明显增加,差异均有统计学意义(均为P<0.05)。缺氧条件下,与对照组相比各实验组IL-1β、IL-6、IL-8及TNF-α的mRNA和蛋白表达在各时间点均明显增加,差异均有统计学意义(均为P<0.05)。结论无论在正常氧含量还是缺氧条件下,贝伐单抗均可刺激纤维化相关炎症因子的表达增加,此部分炎症因子可能参与了抗新生血管内皮生长因子治疗后的新生血管纤维化过程。Objective To investigate the effects of bevacizumab on the expressions of fibrosis-related inflammatory mediators in human umbilical vein endothelial cells（HUVEC）,and further clarify the mechanism of fibrosis after the treatment of bevacizumab.Methods HUVEC were cultured under normal oxygen and hypoxia conditions,respectively.And bevacizumab were added into the culture medium.Cells were divided into 5groups according to incubation period：0 hour（control）,6 hours,12 hours,24 hours and48 hours.The mRNA and protein levels of interleukin（IL）-1β,IL-6,IL-8,and tumor necrosis factor（TNF）-α were evaluated by SYBR green real-time PC R and ELISA,respectively.Results Under normoxic conditions,bevacizumab significantly increased the secretion of IL-6 and IL-8 at 6 hours,12 hours,24 hours and 48 hours,and IL-1β and TNF-α were only increased obviously at 12 hours,24 hours and 48 hours after the treatment of bevacizumab（all P〈0.05）.Bevacizumab significantly increased the secretion of IL-1β,IL-6,IL-8,and TNF-α under hypoxic conditions at 6 hours,12 hours,24 hours and 48 hours（all P0.05）.Conclusion Treatment of HUVEC with bevacizumab significantly increase the secretion of fibrosis-related inflammatory mediators.Those inflammatory cytokines may be involved in the fibrosis process after anti-vascular endothelial growth factor treatment.

关 键 词：贝伐单抗 血管纤维化 炎症因子 人脐静脉内皮细胞 

分 类 号：R285.5[医药卫生—中药学]