LY294002联合奥曲肽对bFGF诱导的兔晶状体上皮细胞增殖的影响  

Effects of LY294002 and octreotide on bFGF-induced proliferation of lens epithelial cells in rabbits

作　　者：冯希敏[1] 张凤妍[2] 

机构地区：[1]新乡市中心医院眼科,河南省新乡市453000 [2]郑州大学第一附属医院眼科,河南省郑州市450052

出　　处：《眼科新进展》2015年第11期1036-1038,1043,共4页Recent Advances in Ophthalmology

摘　　要：目的探讨LY294002联合奥曲肽对碱性成纤维细胞生长因子(basic fibroblast growth factor,bFGF)诱导的兔晶状体上皮细胞(lens epithelial cells,LECs)增殖的影响。方法兔眼LECs经原代和传代培养后,共分为4组:空白对照组、增殖对照组、实验药物组、增殖药物组。空白对照组使用仅含无血清DMEM培养;增殖对照组使用加bFGF(10 mg·L-1)的无血清DMEM;实验药物组培养时在不加bFGF增殖的情况下分别单独应用10-5 mol·L-1 LY294002、单独应用10-9 mol·L-1奥曲肽和联合应用10-5 mol·L-1 LY294002与10-9 mol·L-1奥曲肽来培养;增殖药物组在加bFGF增殖的情况下分别单独应用10-5 mol·L-1LY294002、单独应用10-9 mol·L-1奥曲肽和联合应用10-5 mol·L-1LY294002和10-9 mol·L-1奥曲肽进行细胞培养,每组重复5次。各组分别培养48 h。MTT比色法测定吸光度(A值)分析各组细胞的生长抑制率,流式细胞仪检测各周期细胞的百分率。结果增殖对照组与空白对照组相比,吸光度A值升高(P<0.05);实验药物组中各组分别与空白对照组相比,吸光度A值均有不同程度下降(均为P<0.05);增殖药物组中各组分别与增殖对照组相比,吸光度A值明显下降(均为P<0.05);生长抑制率与吸光度A值趋势相同。经流式细胞仪分析,实验药物组中联合用药组与两个单独用药组相比G0/G1期细胞比例增高,S期细胞比例降低(均为P<0.05);增殖药物组中,联合用药组与两个单独用药组相比也出现G0/G1期细胞比例增高,S期细胞比例降低(均为P<0.05)。结论 LY294002联合奥曲肽较单独用药对LECs的增殖抑制作用更强。这为临床筛选药物防治后发性白内障提供依据。Objective To investigate the proliferation inhibition effects of LY294002 and octreotide on rabbit lens epithelial cells（LEC） induced by basic fibroblast growth factor（bFGF）. Methods LEC were isolated from rabbit, primarily cultured and passaged, divided into four groups：Blank control group, positive control group, drugs groups, positive drugs groups. LEC was cultured in free-serum DMEM as blank control group, bFGF（ 10 mol·L^-1 ） was used to induce the proliferation of LEC in posi- tive control group. The drugs groups were given only 10^-5 mol·L^-1 LY294002 ,only l0^-9 mol·L^-1 octreotide and 10^-5 mol·L^-1 LY294002 ＋ 10^-9 mol·L^-1 octreotide,rspective- ly. The positive drugs groups were used only 10^-5 mol·L^-1 LY294002,only 10^-9 mol·L^-1 octreotide and 10^-5 mol·L^-1 LY294002 ＋ 10 9mol·L^-1 octreotide,the bFGF（ 10 mg · L^- 1 ） was used to induce the proliferation of LEC, Then cultured for 48 hours. The absorbancy（A value） was detected by methyl thiazolyl tetrazolium（ MTT） for the as- sessment of inhibiting rate of LEC in different groups, and percentage of LEC in differ- ent cell cycles was tested and analyzed with flow cytometry. Results Compared with blank control group, the A value was elevated in positive control group （P 〈 0.05 ）. Com- pared with blank control group ,the A value were obviously decreased to different extent in drugs groups （ all P 〈 0.05 ）. The more obvious descend in A values was seen in posi- tive drugs groups compared with positive control groups （ all P 〈 0.05 ）. In positive drugs group, compared with the octreotide ＋ bFGF group and the LY294002 ＋ bFGF group, the A values of the combined drug ＋ bFGF group were all decreased （ all P 〈 0.05 ）. The in- hibiting rate and the A value showed the same tendency. In drugs groups, compared with only drugs,the percentage of LEC of the combined drugs groups in G0/G1 phasewas increased（ all P 〈0.05） ,and that in S phase was decreased compared with only drugs groups（ all

关 键 词：LY294002 奥曲肽 晶状体上皮细胞 后发性白内障 

分 类 号：R594.8[医药卫生—内科学]