分枝虫草液体培养基筛选及其菌丝成分分析  

Optimization of the Liquid-Culture Medium of Cordyceps ramosa and Composition Analysis of Mycelium

作　　者：林敏[1] 宋斌[1] 黄浩[1] 李挺[1] 沈亚恒[1] 

机构地区：[1]广东省微生物研究所,省部共建华南应用微生物国家重点实验室,广东省菌种保藏与应用重点实验室,广东省微生物应用新技术公共实验室,广东广州510070

出　　处：《中国食用菌》2015年第6期17-21,共5页Edible Fungi of China

基　　金：广东省科技项目(2011B020303003);广东省科技成果转化项目(2012NL031);广州市科技项目(201300000031);中国科学院微生物研究所真菌学国家重点实验室项目(SKLM 2013-2015)

摘　　要：以分枝虫草(Cordyceps ramosa)菌丝生物量作为指标,研究不同碳源和氮源对分枝虫草液体培养的影响,采用正交设计试验法筛选最佳液体培养基配方,并分析菌丝主要营养成分。结果表明,分枝虫草液体培养基的最佳碳源为甘油,最佳氮源为硝酸钾,最佳配方是:甘油35 g·L-1、硝酸钾12 g·L-1、酵母浸膏12 g·L-1、麦芽浸出物10 g·L-1、H2O 1 L;其菌丝的粗蛋白、粗脂肪和总灰分含量分别为32.90%、1.20%和5.89%,其腺苷、多糖和虫草酸的含量分别为0.805%、5.89%和5.61%;含有17种氨基酸,总含量为23.97%;微量元素铁、锰、铜、锌和硒含量分别为76.70 mg·kg-1、5.10 mg·kg-1、3.70 mg·kg-1、33 mg·kg-1和0.176 mg·kg-1,重金属铅、砷、汞的含量均符合国家食用菌相关标准。分枝虫草的液体培养可作为分枝虫草开发利用的重要途径。Effects of carbon and nitrogen sources on the optimization of liquid-culture medium for Cordyceps ramosa mycelial biomass, and the optimum liquid medium screening by the orthogonal design, and the main components of mycelium were ana- lyzed. The results showed that the optimal carbon and nitrogen sources were glycerol and KNO3 respectively, and the optimal medium were glycerol 35 g·L^-1, KNO3 12 g·L^-1, yeast extract fermentation 12 g·L^-1, malt extract 10 g·L^-1 and H2O 1 L. The mycelium contained crude protein （32.9%）, crude fat （1.2%） and ash （5.89%）. The contents of adenosine, polysaccharide, and cordycepic acid were 0.805%, 5.89% and 5.61%. And 17 kinds of amino acids （23.97%） were detected from the myceli- urn. The microelement levels, such as Fe, Mn, Cu, Zn, Se, were 76.70 mg·kg^-1, 5.10 mg·kg^-1, 3.7 mg·kg^-1, 33 mg·kg^-1 and 0.176 mg·kg^-1, respectively. Levels of the heavy metals, such as Pb, As and Hg, met the edible fungus safety standard levels specified by the Ministry of Health of the People＇ s Republic of China. The results could provide important way for the develop- ment and utilization of C. ramosa.

关 键 词：分枝虫草 液体培养基 正交试验 成分分析 

分 类 号：S567.3[农业科学—中草药栽培]