内皮素-2促进体外培养的绵羊皮肤黑色素细胞增殖及黑色素生成  

Endothelin-2 Promotes Ovine Skin Melanocytes Proliferation and Melanin Melanogenesis in Vitro

作　　者：秦以龙 董常生[1] 庞亚妙[1] 崔玉琮 赵园园[1] 耿建军[1] 

机构地区：[1]山西农业大学动物科技学院,山西太谷030801

出　　处：《中国生物化学与分子生物学报》2015年第11期1199-1205,共7页Chinese Journal of Biochemistry and Molecular Biology

基　　金：国家高技术研究发展计划(863计划)(No.2013AA102506);公益性行业(农业)科研专项(201303119)资助基金项目;山西省自然科学基金(2012011046-6)项目~~

摘　　要：内皮素(endothelin,ET)及其受体在黑色素细胞成熟分化时起着有效的促进作用.然而,内皮素-2(ET-2)在黑色素生成的作用方面,还处于争论中或报道不一致.在此,我们研究ET-2对体外培养的绵羊皮肤黑色素细胞增殖和黑色素生成的影响.比较实验组ET-2(1,10,100 nmol/L)与空白对照组,通过MTT法和Ando等的方法检测出黑色素细胞的增殖率和黑色素含量显著增加.荧光定量PCR和Western印迹分别检测出内皮素受体B(Bdnrb);酪氨酸激酶受体(Kit);酪氨酸酶(Tyr);酪氨酸相关蛋白-1(Tyrp-1)基因的mRNA水平及蛋白水平的相对表达量显著增加.这些数据表明,ET-2可能促进绵羊的皮肤黑色素细胞增殖和黑色素生成.Endothelin（ ET） and its receptor have been shown to play roles in the melanocyte maturity.However,the reports on the function of ET-2 in melanogenesis are debated. Herein,we investigated the effect of ET-2 on ovine skin melanocyte proliferation and melanogenesis. MTT method and Ando methods showed that the cell proliferation rates and melanin levels were significantly increased in melanocytes treated by ET-2（ 1 to 100 nmol / L） compared with the control（ untreated） cells. Furthermore,real-time quantitative PCR and Western blotting revealed that the levels of both mRNA and protein of the Bdnrb,Tyr,Kit and Tyrp-1 genes were significantly increased. These data suggest that ET-2 might promote proliferation of ovine skin melanocytes and melanogenesis.

关 键 词：内皮素-2 黑色素细胞 内皮素受体B 酪氨酸激酶受体 酪氨酸酶 酪氨酸相关蛋白-1 黑色素 

分 类 号：S826.95[农业科学—畜牧学]