小麦品种云麦53抗条锈基因的分子标记定位  

Molecular Mapping of Stripe Rust Resistance Gene in Wheat Cultivar Yunmai 53

作　　者：刘鹏[1,2] 陈璨[1] 卢家玲[1] 白斌[3] 何中虎[1,4] 夏先春[1] 

机构地区：[1]中国农业科学院作物科学研究所/国家小麦改良中心,北京100081 [2]山东省德州市农业科学研究院,山东德州253015 [3]甘肃省农业科学院小麦研究所,甘肃兰州730070 [4]国际玉米小麦改良中心(CIMMYT)中国办事处,北京100081

出　　处：《麦类作物学报》2015年第11期1483-1488,共6页Journal of Triticeae Crops

基　　金：国家重点基础研究发展计划(973计划)项目(2013CB127700);国家自然科学基金项目(31261140370);国家小麦产业体系项目(CARS-3-1-3)

摘　　要：云麦53是以CIMMYT优异品系为亲本,通过基因聚合选育的高产、抗病、广适性小麦新品种。为明确云麦53的抗条锈病遗传基础,利用我国当前流行的条锈菌生理小种条中29(CYR29)对云麦53与辉县红杂交后代F1、F2和F2∶3群体进行苗期抗病性鉴定和遗传分析,并利用分子标记对抗条锈病基因进行定位。遗传分析表明,云麦53对CYR29的抗性由1对显性基因控制,暂定名为YrYM53。利用分子标记对F2和F2∶3群体进行检测,发现3个与YrYM53紧密连锁的分子标记(barc61、barc240和AF1/AF4),与YrYM53的遗传距离分别为3.3cM、3.9cM和7.6cM,说明该基因位于1B染色体的长臂上。根据基因来源和抗谱分析,YrYM53与1B或1BL/1RS染色体上已知抗条锈病基因Yr9、Yr10、Yr15、Yr24/Yr26、YrZH84.2和YrCN17不同,可能是一个新的抗病基因。Yunmai 53 is an elite wheat cultivar with high yield, stripe rust resistance and wide ecological adaptability,and it was bred by gene pyramiding using CIMMYT wheat lines. In order to reveal the genetic basis of Yumai 53 resistant to stripe rust,F1 ,F2 and F2： 3 populations derived from the cross of Huixianhong/Yunmai 53 were inoculated with Chinese Puccinia striiformis f. sp. tritici race CYR29 in the greenhouse for the identification of seedling resistance gene in Yunmai 53. Results indicated that Yunmai 53 carried a single dominant resistance gene to CYR29, temporarily designated as YrYM53. Using molecular markers to analyze F2 and F2： 3 populations, YrYM53was mapped on chromosome 1BL,closely linked to markers barc61, barc240 and AF1/AF4 with genetic distances of 3, 3,3. 9 and 7.6 cM,respectively. Pedigree analysis and reactions to Pst races suggested that YrYM53is different from previously identified genes Yr9, Yr10, Yr15, Yr24/Yr26, YrZH84. 2and YrCN17on chromosomes 1B or 1BL/1RS.

关 键 词：小麦 条锈病 抗性基因 分子标记 

分 类 号：S512.1[农业科学—作物学] S330