荧光定量PCR检测戊肝病毒的应用价值探究  

Exploration of the application value of fluorescent quantitative PCR on detection of hepatitis E virus

作　　者：陈欣 梁雨然 曹誉龄 李进春 李佩菁 曹昊 杨颖馨 

机构地区：[1]成都市锦江区疾病预防控制中心,四川成都610021 [2]美国科罗拉多州立大学,科罗拉多州科林斯堡80526

出　　处：《现代预防医学》2015年第22期4162-4164,共3页Modern Preventive Medicine

摘　　要：目的探讨荧光定量PCR检测HEV RNA的应用价值。方法从110 535份血清标本中,随机抽取标本200份,分别进行ELISA检验和荧光定量PCR分析。结果 69例ELISA阳性的标本经荧光定量PCR检测均为阳性,31例ELISA可疑的标本经荧光PCR检测也均为阳性,100例ELISA阴性的标本中有2例经荧光定量PCR检测结果为阳性。经Kappa检验,Kappa值为0.72,可认为2种方法检测结果具有中度一致性。采用Stuart-Maxwell检验,结果显示PCR与ELISA检出3种情况的边际概率不相等(χ2=33,P=0.00,P<0.05),提示2种方法检测结果存在差异,即荧光PCR的阳性检出率更高。结论荧光定量PCR检测HEV RNA能客观地反映HEV感染、复制及病程变化,修正或补充对ELISA测定结果的判定和解释,有利于HEV感染的早期诊断。Objective The objective of the study was to explore the application value of fluorescent quantitative PCR（FQ-PCR） on detection of hepatitis E virus（HEV） RNA. Methods 200 samples were randomly selected from 110535 serum specimens, and were analyzed ELISA and FQ-PCR. Results All 69 ELISA-positive, 31 ELISA-ambiguous, and 2 out of 100 ELISA-negative samples were tested positive of HEV RNA by FQ-PCR. The Kappa value was 0.72, indicating that results of the two methods were of moderate consistency. Stuart-Maxwell test showed that PCR and ELISA resulted in different marginal probabilities of the three conditions（χ^2=33, P=0.00, P〈0.05）, suggesting that results of the two methods were statistically different, and that FQ-PCR had higher positive rate. Conclusion Detection of HEV RNA by FQ-PCR can objectively reflect the infection, replication, and course changes of HEV, and can correct or supplement the results of ELISA assays; therefore, is conducive to early diagnosis of HEV infection.

关 键 词：荧光定量聚合酶链反应 酶联免疫吸附测定实验 戊型肝炎病毒 RNA 

分 类 号：R319[医药卫生—基础医学]