葛花解酲方对肝癌细胞原位移植瘤小鼠抗氧化作用的影响  

Antioxidant effects of Gehua Jie Cheng Fang on orthotopic allograft HCC mice

作　　者：王镜辉[1] 唐东昕[1] 黄慧[1] 罗莉[1] 龙奉玺[2] 李军[2] 王定雪[1] 杨柱[2] 

机构地区：[1] 贵阳中医学院第一附属医院 贵州550001 [2] 贵阳中医学院

出　　处：《北京中医药大学学报》2015年第10期690-693,I0002,I0003,共6页Journal of Beijing University of Traditional Chinese Medicine

基　　金：国家自然科学基金项目(No.81160417;No.81460697);贵州省研究生工作站计划项目(No.黔教研合JYSZ字[2014]018);贵州省科学技术厅-贵阳中医学院科学技术联合基金项目(No.黔科合LH字[2014]7336号)

摘　　要：目的观察葛花解酲方对肝癌细胞原位移植瘤小鼠抗氧化作用的影响。方法采用H22小鼠腹水瘤细胞原位移植瘤动物模型,对癌变小鼠进行治疗。通过HE染色切片观察小鼠肝脏的组织和细胞形态变化。ELISA检测血清超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)、丙二醛(MDA)的含量并观察免疫组化染色的表达情况。结果 H22腹水瘤细胞诱发的原位移植瘤小鼠肝脏可出现无限增殖、坏死等病理变化。葛花解酲方可调节SOD、MDA、GSH-Px的表达水平,在一定程度上具有抗氧化损伤作用,进而减缓模型小鼠癌变进程。结论葛花解酲方对H22小鼠腹水瘤细胞诱发的肝脏肿瘤有确切的疗效,其机制可能与抗氧化作用的激活有关。Objective To observe the antioxidant effects of Gehua JieCheng Fang （ Kudzuvine Flower Formula for hang-over symptoms） on the orthotopic allograft mice.Methods Orthotopic allograft models were established using H22 ascites tumor cells.Cancerous mice were then treated.Pathological changes of the hepatic lobe were microscopically observed after HE staining.Levels of superoxide dismutase （ SOD ） , Glutathione peroxidase （ GSH-Px ） , and Malondialdehyde （ MDA ） in the serum were determined by using ELISA methods.Expression levels of SOD, GSH-Px, and MDA in hepatic tissues were compared.Results Liver of the orthotopic allograft mice induced by H22 ascites tumor cells presented with pathological changes of unlimited proliferation and necrosis. Gehua JieCheng Fang affected the expression of SOD, MDA, and GSH-Px, which demonstrated the antioxidant effects.It could slow down carcinogenesis in these mice.Conclusion Gehua JieCheng Fang seems to have therapeutic effects on mice with hepatocarcinoma induced by H22 ascites cells.Its mechanism is related to the activation of antioxidation.

关 键 词：葛花解酲方 肝癌 原位移植瘤 抗氧化 小鼠 

分 类 号：R285.5[医药卫生—中药学]