木薯干旱胁迫响应基因MeGSTU7自然变异分析  

Natural Variation Analysis of MeGSTU7, One Drought Responsive Gene of Cassava(Manihot esculenta)

作　　者：邓德力[1] 王斌[1,2] 曾长英[1] 郭鑫[1] 彭明[1] 

机构地区：[1]海南大学农学院,海南海口570228 [2]中国热带农业科学院热带生物技术研究所农业部热带作物生物学与遗传资源利用重点实验室,海南海口571101

出　　处：《热带作物学报》2015年第11期1986-1993,共8页Chinese Journal of Tropical Crops

基　　金：国家“863”计划项目(No.2012AA101204-2);国家国际科技合作专项(No.2013DFA32020);海南省重大科技项目子课题(No.ZDZX2013023-1);海南省自然科学基金(No.313075);中央级公益性科研院所基本科研业务费(No.ITBB2015RC01)

摘　　要：在2个木薯品种中检测MeGSTU7基因在不同干旱胁迫阶段的表达量变化,并克隆测序了60个木薯品种的基因区DNA序列,分析基因核苷酸多态性及其自然变异,并将核苷酸多态性与干旱胁迫表型关联分析,挖掘优等位变异。结果表明,干旱胁迫条件下,2个品种的MeGSTU7的表达量均上调。MeGSTU7基因区核苷酸变异丰富,共有23个SNP位点,A/G突变为主;外显子区总计10个同义突变,12个非同义突变;外显子区在品种资源群体中有4种主要单倍型,所有的单倍型分为两大类,分子进化分析表明,MeGSTU7的外显子区的两端受到很强的正选择作用;Q+K+MLM混合线性模型关联分析结果表明,1个Indel和2个SNP与干旱胁迫下地上部鲜重耐旱系数显著关联,并筛选得到了优等位变异。Glutathione-S-transferase（GST） plays an important role in glutathione conjugation reaction and involves in many physiological and biochemical reaction in plants including stress resistance, primary metabolism and cellular signal transduction. MeGSTU7 codes GST in cassava（Manihot esculenta crantz）. In order to research the function of MeGSTU7 in drought-resisting, the expression quantity of MeGSTU7 was detected in two cassava varieties which are tolerant and sensitive to water stress in different degrees of drought stress. MeGSTU7 was cloned from 60 cassava varieties and sequenced. Sequceces were analyzed to study the single nucleotide polymorphism（ SNP） and the natural variation. Correlation analysis between single nucleotide polymorphism and drought phenotype was made to select the excellent allelic variations. The results indicated that the expression of MeGSTU7 in two cassava varieties were up-regulated under drought stress. Nucleotide polymorphism in the gene regions of MeGSTU7 was abundant with 23 SNP among which the A/G mutation type was the major. There were10 synonymous changes and 12 replacement changes in exon regions. All of the haplotypes were divided into two types among which 4 haplotypes were the major in population. Molecular evolution showed that intense positive selection function at the ends of the exon in MeGSTU7. By Q＋K＋MLM mixed linear model, one Indel and 2 SNP were significantly associated with the fresh weight of aerial parts in the stress of drought and excellent allelic variations were selected. In conclusion, this paper lays a good foundation for further research of the function of MeGSTU7 in drought resistant and the selection of excellent allelic variations.

关 键 词：谷胱甘肽转移酶 MeGSTU7 木薯 抗旱 自然变异 单倍型 

分 类 号：S533[农业科学—作物学]