小分子单克隆抗体的制备与敲除技术研究进展  

Progress in preparation of small monoclonal antibodies of knock out technique

作　　者：刘菁[1] 毛新民[2] 李琳琳[1] 李新霞[3] 王烨[1] 兰怡[4] 

机构地区：[1]新疆医科大学基础医学院,新疆乌鲁木齐830011 [2]新疆医科大学中医学院,新疆乌鲁木齐830011 [3]新疆医科大学科研中心,新疆乌鲁木齐830011 [4]新疆医科大学附属中医医院,新疆乌鲁木齐830011

出　　处：《中国中药杂志》2015年第19期3737-3741,共5页China Journal of Chinese Materia Medica

基　　金：NSFC-新疆联合基金项目(U1303223)

摘　　要：随着单克隆抗体技术应用的愈加广泛,其生产技术也日臻完善。小分子单克隆抗体技术也日益成为人们研究的热点课题。中药小分子单克隆抗体技术的应用也日益广泛,这门技术给中药有效成分的敲除提供有力的技术支持。该文就小分子单克隆抗体制备与敲除技术进行了综述。就其制备过程中的几个步骤,如:半抗原的制备,半抗原与载体偶联,人工抗原的鉴定及偶联比率的测定,动物免疫与单克隆杂交瘤细胞株的建立,单克隆抗体的大规模制备;小分子单克隆抗体的敲除技术的免疫亲和色谱柱的方法进行了详尽的阐述。笔者相信这门技术会使得中药的研究上更高一个层次,并且提高中药研究的国际化水平。With the application of monoclonal antibody technology more and more widely, its production technology is becoming more and more perfect. Small molecule monoclonal antibody technology is becoming a hot research topic for people. The application of traditional Chinese medicine small molecule monoclonal antibody technology has been more and more widely, the technology for effective Chinese medicine component knockout provide strong technical support. The preparation of monoclonal antibodies and small molecule knockout technology are reviewed in this paper. The preparation of several steps, such as ： in the process of preparation of antigen, hapten carrier coupling, coupling ratio determination and identification of artificial antigen and establishment of animal immunization and hybridoma cell lines of monoclonal antibody, the large-scale preparation ; small molecule monoclonal antibody on Immune in affinity chromatography column method is discussed in detail. The author believes that this technology will make the traditional Chinese medi- cine research on a higher level, and improve the level of internationalization of Chinese medicine research.

关 键 词：半抗原 人工抗原 偶联比率 单克隆抗体 动物免疫 杂交瘤细胞 免疫亲和色谱柱 

分 类 号：R392[医药卫生—免疫学]