人参灰霉菌和人参黑斑菌对人参总皂苷的化学趋向性响应研究  

Research of chemotaxis response of Botrytis cinerea and Alternaria panax on total ginsenosides

作　　者：匙坤 许永华[1] 雷锋杰[1] 尹敏晶[1] 王壮[1] 张爱华[1] 张连学[1] 

机构地区：[1]吉林农业大学中药材学院吉林省人参工程技术研究中心,吉林长春130118

出　　处：《中国中药杂志》2015年第19期3742-3747,共6页China Journal of Chinese Materia Medica

基　　金：国家自然科学基金项目(31100239;31200224;31470420);吉林省世行贷款农产品质量安全应用研究项目(2011-Z25);质检公益性行业项目(201410297);吉林省科技发展计划项目(20130206030YY;20140520159JH)

摘　　要：采用平板法和孢子悬滴萌发法分别测定了人参灰霉菌和人参黑斑菌在3种趋化参数(浓度、温度和p H)下对人参总皂苷的化学趋向性响应。结果表明,人参灰霉菌在20℃和p H 6的培养条件下,对20 mg·L-1的人参总皂苷表现出较强的化学趋向性,趋化移动指数(CMI)为1.293 0,趋化生长速率(CGR)为0.476 0,孢子萌发率(SGR)为53%,菌丝干重(DWM)为0.452 6 g·L-1;而人参黑斑菌则在25℃和p H 6的培养条件下,对2 mg·L-1的人参总皂苷表现出较强的化学趋向性,趋化移动指数(CMI)为1.235 4,趋化生长速率(CGR)为0.537 0,孢子萌发率(SGR)为67%,菌丝干重(DWM)为0.494 8 g·L-1。显示人参灰霉菌和人参黑斑菌分别对低(2 mg·L-1)、中(20 mg·L-1)浓度的人参总皂苷具有正向的化学趋向性响应,而且受此趋化影响下的孢子萌发率、菌丝生长速率及菌丝生长量都得到了显著提高,但随皂苷浓度的升高而降低。In this paper, three kinds of chemotactic parameters （concentration, temperature and pH） were determined by plate as- say and spore germination method to research the ehemotacfic response of Botrytis cinema and Alternaria panax, and their spores on total ginsenosides. The results showed that Botrytis cinerea had strong ehemotaetic response at the mid-concentration of total ginsenosides （ cultivation temperature was 20 ℃ and pH value was 6）, and the data of chemotactic migration index （CMI） was 1. 293 0, ehemotactic growth rate （CGR） was 0. 476 0, spore germination rate （SGR） was 53%, and dry weight of mycelial （DWM） was 0. 452 6 g · L -1; however, Alternaria panax had strong ehemotaetic response at the low-concentration of total ginsenosides （ cultivation temperature was 25 ℃ and pH value was 6）, and the data of chemotactic migration index （CMI） was 1. 235 4, chemotactic growth rate （CGR） was 0. 537 0, spore germination rate （SGR） was 67%, and dry weight of mycelial （DWM） was 0. 494 8 g · L-1. The results indicated that the low and middle concentration （2, 20 mg · L-1 ） of total ginsenosides had significant promoting effect on ehemotactic response of these two pathogens, and the spore germination, mycelial growth rate, dry weight of myeelial of them were also significantly improved by this chemotactic response, whereas it decreased as the increase of total ginsenosides concentration.

关 键 词：人参灰霉菌 人参人参黑斑菌 人参总皂苷 化学趋向性 

分 类 号：S435.675[农业科学—农业昆虫与害虫防治]