酸枣仁皂苷回流提取工艺的响应面优化  

Optimization of reflux extraction of jujuboside from Ziziphi spinosae semen by central composite design-response surface methodology

作　　者：祝洪艳[1] 张力娜[1] 林海成 王月珍[2] 王国丽[1] 赵岩[1] 郜玉钢[1] 张连学[1] 

机构地区：[1]吉林农业大学中药材学院,吉林长春130118 [2]长春医学高等专科学校,吉林长春130031

出　　处：《西北农林科技大学学报（自然科学版）》2015年第11期207-213,220,共8页Journal of Northwest A&F University(Natural Science Edition)

基　　金：国家公益性行业(农业)科研专项(201303111);科技部科技支撑项目(2011BAI03B01);吉林省科技发展计划项目(20126046);吉林省世行贷款农产品质量安全项目(2011-Z25);吉林省教育厅"十二五"项目(2014第60号)

摘　　要：【目的】采用中心组合试验-响应面法优选酸枣仁皂苷的最佳回流提取工艺,为酸枣仁皂苷提取的工业化生产提供依据。【方法】采用HPLC-UV检测酸枣仁皂苷A和B的含量,用乙醇回流提取法,以乙醇体积分数(0,30%,50%,70%,80%,95%)、液固比(8∶1,10∶1,12∶1,15∶1,20∶1)、提取时间(1,1.5,2,2.5,3h)和提取次数(1,2,3,4)为考察因素,筛选各因素对酸枣仁皂苷提取影响的取值范围,在此基础上进行中心组合试验,用SAS(9.2)软件建立预测模型,响应面分析最佳提取条件。【结果】响应面分析优选得到的酸枣仁皂苷最佳提取工艺为:体积分数66%乙醇,液固比12.5∶1,回流提取3次,每次1.8h。在此条件下,酸枣仁皂苷A和B含量之和平均为1.21mg/g,与模型预测值(1.30mg/g)接近。【结论】用中心组合设计-响应面法优选的酸枣仁皂苷A和B提取工艺可信度高,皂苷含量验证值与模型预测值接近,模型预测效果好,工艺稳定,可用于指导酸枣仁皂苷的工业化提取生产。【Objective】The reflux extraction of jujuboside from Ziziphi spinosae semen was optimized by central composite design-response surface methodology to provide basis for industrial extraction of jujuboside from Ziziphi spinosae semen.【Method】The contents of jujuboside A and B were detected by HPLCUV,using ethanol extraction.Different ethanol concentrations（0,30%,50%,70%,80%,and 95%）,solvent-to-solid ratios（8∶1,10∶1,12∶1,15∶1,and 20∶1）,extraction times（1,1.5,2,2.5,and 3h）and extraction frequencies（1,2,3,and 4）were tested to screen value ranges.Then,central composite design（CCD）-response surface methodology was used and a predictive model was developed by SAS（9.2）software to determine the optimum extraction conditions.【Result】The optimum reflux extraction conditions were：66% ethanol,solvent-to-solid ratio 12.5∶1,and extracted for 1.8hand 3times.The average content of jujuboside A and B was 1.21mg/g,which was close to the model prediction（1.30mg/g）.【Conclusion】CCD-response surface methodology for optimizing the reflux extraction of jujuboside A and B was reliable,and the detected content of jujuboside was close to the predicted value.The predicted extraction process was also stabled and could be used to direct the industrialized extraction of jujuboside.

关 键 词：酸枣仁皂苷A 酸枣仁皂苷B 提取工艺 中心组合设计-响应面法 

分 类 号：S665.109.9[农业科学—果树学] R284.2[农业科学—园艺学]