补脑止痫散对戊四氮慢性点燃癫痫大鼠模型GABA及其受体GABAα表达的影响  

Effects of Bunao Zhixian San on expression of γ-aminobutyric acid(GABA) and GABAα in epilepsy rats

作　　者：徐先伟[1] 程为平[1] 

机构地区：[1]黑龙江中医药大学

出　　处：《中医临床研究》2015年第29期1-2,5,共3页Clinical Journal Of Chinese Medicine

摘　　要：目的:揭示补脑止痫散对戊四氮(PTZ)慢性点燃癫痫大鼠模型γ-氨基丁酸(GABA)及其受体亚单位α(GABAα)表达的影响。方法:1选取60只健康SD大鼠,分成6组(空白对照组、癫痫模型组、丙戊酸钠组、补脑止痫散小剂量组、补脑止痫散中剂量组、补脑止痫散高剂量组)。2采用连续小剂量腹腔注射戊四氮(PTZ)的方法,建立癫痫大鼠模型(慢性点燃)。凡是获得III级以上发作后,均停止给药。后采用免疫组织化学法,检测各组大鼠海马区GABA及其GABAα的表达。结果:除空白对照组无癫痫样发作外,其余各组都出现Racine分级中的III-IV痫样发作表现。通过免疫组织化学法,检测各组大鼠海马区GABA及其GABAα的表达。丙戊酸钠组、补脑止痫散高剂量组GABA及GABAα表达明显上调,与模型组比较,差异显著,具有统计学意义(P<0.05)。结论:补脑止痫散对戊四氮慢性点燃癫痫大鼠模型海马区内抑制性递质及受体有明显的上调作用,从而起到保护脑组织、缓解癫痫症状,进而减轻由癫痫导致的脑损伤的作用。Objectives： To study the effects of Bunao Zhixian San on expression ofγ-aminobutyric acid （GABA） and GABAα in epilepsy rats. Methods： 60 SD rats were randomly divided into 6 groups, control group, epileptic rat model group, sodium valproate group, low dose of Bunao Zhixian San therapy group, middle dose of Bunao Zhixian San therapy group and high dose of Bunao Zhixian San therapy group. Rat kindling model of epilepsy was established by PTZ injection. By immunohistochemistry method, the protein expression of GABA and GABAα in hippocampus of rats was detected. Results： The occurrence and seizure level were evaluated by Racine’5 methods. The rats with III to V seizure level were chosen for the following studies. The expression of GABA and GABAα were increased significantly in the sodium valproate group and the high dose of Bunao Zhixian San therapy group. There were significant difference compared with model group （P〈0.05）. Conclusions： Bunao Zhixian San has significantly increased role in excitatory neurotransmitter and receptor, to protect the brain tissue, alleviate the symptoms of epilepsy, and reduce the damage caused by epilepsy.

关 键 词：补脑止痫散 戊四氮 癫痫大鼠 γ-氨基丁酸 氨基丁酸受体亚单位α 

分 类 号：R286[医药卫生—中药学]