DNA条形码试剂盒检测技术在墨天牛鉴定中的应用  

The application of DNA barcoding identification kit technology to Monochamus Dejean

作　　者：陈梦义[1,2] 徐梅[3] 钱路[3] 安榆林[3] 郝德君[1,2] 

机构地区：[1]南京林业大学南方现代林业协同创新中心,江苏南京210037 [2]南京林业大学林学院,江苏南京210037 [3]江苏出入境检验检疫局,江苏南京210001

出　　处：《南京林业大学学报（自然科学版）》2015年第6期7-11,共5页Journal of Nanjing Forestry University：Natural Sciences Edition

基　　金："十二五"国家科技支撑计划(2012BAK11B03);国家自然科学基金项目(31170606);江苏高校优势学科建设工程资助项目(PAPD)

摘　　要：为了对进境口岸截获的俄罗斯木材中的墨天牛属(非中国种)害虫进行准确、快速鉴定,笔者利用DNA条码试剂盒检测技术,基于墨天牛线粒体COⅠ(线粒体细胞色素氧化酶亚基I)基因设计一对巢式引物,对截获墨天牛属中的8个种进行COⅠ扩增,将扩增序列上传至有害生物信息网站,依据碱基位点在不同种间的排列和组成的差异,获得不同种在相应位点上的特有碱基,即标记性碱基,以此作为区分墨天牛种的依据。通过这种墨天牛属DNA条码试剂盒检测技术,可为口岸检疫鉴定提供有效的技术手段。In order to rapidly and accurately identify theMonochamusof wood from Russian, we employed DNA barcodedetection kit technology and designed a pair of nested primers based on mitochondrialCOⅠ gene（mitochondrial cyto-chrome oxidase subunit I）. Furthermore,COⅠ gene sequence of eightMonocharnusspecies was amplificated, and up-loaded to pest information site. According to differences of arrangement and composition among these species at basesites, species unique bases was got in the corresponding sites, which is named specific site to distinguish this species. Inthis way, we developed the DNA barcoding identification kit technology toMonochamusspecies, which will provide anew way for port quarantine identification.

关 键 词：墨天牛属 COⅠ基因 DNA条形码试剂盒 标记性碱基 

分 类 号：S763[农业科学—森林保护学]