不同剂量活血、破血药对AS小鼠主动脉病理变化及斑块内CD147表达的影响  

The Effect of Blood-activating and Blood-breaking Medicines on Aortic Histopathological Changes and the Expression of CD147 in Atherosclerotic Plaque of ApoE Gene Deficient Mice AS Models

作　　者：汪典[1] 刘亚娟[1] 石协桐 刘丹[3] 罗尧岳[1] 王敏[3] 卢青[3] 陈旭[3] 谢海波[3] 

机构地区：[1]湖南中医药大学,湖南长沙410208 [2]广东省揭阳市人民医院,广东揭阳522000 [3]湖南中医药大学第一附属医院,湖南长沙410007

出　　处：《中国中医急症》2015年第11期1881-1884,1901,共5页Journal of Emergency in Traditional Chinese Medicine

基　　金：国家自然科学基金面上项目(81273752)

摘　　要：目的探究不同剂量活血药(川芎、当归)、破血药(三棱、莪术)对动脉粥样硬化(AS)小鼠主动脉组织病理变化及斑块内CD147基因表达的影响。方法将64只4周龄ApoE基因敲除小鼠制备AS模型,模型制备成功后随机分为6组:模型对照组(模型组)、阿托伐他汀钙组(他汀组)、低剂量活血药组(低活血组)、低剂量破血药组(低破血组)、高剂量活血药组(高活血组)、高剂量破血药组(高破血组)。连续灌胃给药8周后,处理动物。光镜及透射电子显微镜下观察主动脉组织病理变化,利用计算机图像分析系统计算校正斑块面积即斑块面积与血管横截面积的比值(PA/LA);RT-PCR法检测CD147基因mRNA表达水平。结果活血药、破血药均能不同程度改善小鼠主动脉组织病理变化;同时,活血药、破血药均能显著降低斑块内CD147基因表达水平(P<0.01),且活血药高、低剂量组间以及破血药高、低剂量组间存在剂量依赖性(P<0.01),而在相同剂量条件下,破血药作用显著优于活血药(P<0.01)。结论活血药(当归、川芎)、破血药(三棱、莪术)具有抗AS的作用,其作用机制可能与调节斑块内CD147基因表达水平有关。Objective： To observe the effects of different dosages of blood-activating medicines（angelica sinensis and ligusticum wallichii） and blood-breaking medicines（rhizoma sparganii and curcuma zedoary） on pathological changes of aorta and the expression of CD147 in atherosclerotic plaque of apolipoprotein E（ApoE） gene deficient mice models. Methods： 64 ApoE gene deficient mice atherosclerosis（AS） models of four weeks were randomly divided into 6 groups： the model group,atorvastatin group（the statin group）,low dose of blood-activating group（low blood-activating group）,low dose of blood-breaking group（low blood-breaking group）,high dose of blood-activating group（high blood-activating group）,high dose of blood-breaking group（high blood-breaking group）. The mice were killed after 8 weeks′ drug gastric perfusion. The histopathological changes of aorta and the expression of CD147 in atherosclerotic plaque were detected. Results： Blood-activating medicines and blood-breaking medicines could improve the arotic histopathological changes in mice of different degrees and significantly cut CD147 expression in atherosclerotic plaque（P〈0.01）. There was a significant independence between high and low dose blood-activating group and blood-breaking group（P〈0.01）. While blood-breaking medicines showed a better effect than blood-activating medicines with the same dose（P〈0.01）. Conclusion： Blood-activating（angelica sinensis and ligusticum wallichii） and blood-breaking medicines（rhizoma sparganii and curcuma zedoary） show the anti-atherosclerotic effect,whose mechanism may be related to the adjustment of CD147 expression in atherosclerotic plaque.

关 键 词：动脉粥样硬化 APOE基因敲除小鼠 细胞外基质金属蛋白酶诱导因子 活血药 破血药 

分 类 号：R285.5[医药卫生—中药学]