电针百会、神庭穴对MCAO大鼠学习记忆能力及IL-1β、TNF-α表达的影响  

Effect of Electro-acupuncture on Learning and Memory Ability and IL-1β,TNF-α Expression in Rats with Cerebral Ischemic/Reperfusion

作　　者：俞坤强 李晓洁[1] 彭洪卫 卓沛元[1] 张颖铮 林晓敏[1] 林如辉[1] 陶静[1] 陈立典[1,2,3] 

机构地区：[1]福建中医药大学,福建福州350122 [2]福建康复重点实验室,福建福州350122 [3]国家中医药管理局中医康复研究中心,福建福州350122

出　　处：《中国中医急症》2015年第11期1891-1894,1914,共5页Journal of Emergency in Traditional Chinese Medicine

基　　金：科技部国际科技合作项目(2011DFG33240);福建省自然科学基金项目(2015J01335);福建省康复技术协同创新中心资助项目(X2012004-协同)

摘　　要：目的观察电针神庭、百会穴对局灶性脑缺血再灌注大鼠学习记忆的影响,并探讨其可能的机制。方法雄性Sprague-Dawley大鼠45只,采用线栓法制备局灶性脑缺血再灌注大鼠模型,造模成功后随机分为假手术组、模型组和电针组,各15只。电针组大鼠取神庭、百会穴,电针治疗7 d。各组大鼠在造模后第3天开始采用定位航行实验和空间探索实验检测学习记忆能力;观察各组大鼠神经行为学的表现;2,3,5-氯化三苯基四氮唑(TTC)检测脑梗死体积;苏木素-伊红(HE)染色观察海马区神经元破坏和水肿情况;蛋白免疫印迹(Western blotting)法检测大鼠左侧海马白细胞介素-1β(IL-1β)、肿瘤坏死因子α(TNF-α)炎症因子的表达。结果同模型组相比,电针组大鼠学习记忆能力改善(P<0.05),神经行为学评分降低(P<0.05),脑梗死体积减小(P<0.001),海马CA1区神经元破坏情况减轻,IL-1β、TNF-α炎症因子表达降低(P<0.05)。结论电针能改善局灶性脑缺血再灌注大鼠学习记忆能力,其机制可能与减轻脑内神经元的破坏和水肿、抑制IL-1β、TNF-α炎症因子表达相关。Objective： To observe the efficacy of treatment with electro-acupuncture at Baihui（DU20） and Shenting（DU24） on learning and memory ability in rats after cerebral ischemia/ reperfusion（I/R） injury and explore its possible mechanism. Methods： 45 male Sprague-Dawley rats were randomly divided into the sham group（n=15）,the ischemia group（n=15） and the electro-acupuncture group（n=15） after surgery. The latter 2groups were modeled as focal cerebral ischemia/ reperfusion（I/R） injury,and the electro-acupuncture group received electro-acupuncture at Baihui（DU20） and Shenting（DU24） for 7 days. The learning and memory ability were tested by Morris water maze;the cerebral infarction volume was measured by TTC staining and image analysis;neurological deficit score was evaluated;the damage and edema of neuron were detected with hematoxylin-eosin staining,and the protein expression of IL-1β and TNF-α were detected by Western blotting. Results：Compared with the ischemia group,the learning and memory ability improved（P〈0.05）;the neurological deficit score decreased（P〈0.05）;the cerebral infarction volume reduced（P〈0.001）;the neuronal damage reduced,and the expression of IL-1β and TNF-α in hippocampus decreased（P〈0.05） in the electro-acupuncture group. Conclusion： Electro-acupuncture can improve learning and memory ability from cerebral ischemia/ reperfusion（I/R）injury in rats,which may be related with reducing the damage and edema of neuron and inhibiting the expression of IL-1β and TNF-α in hippocampus.

关 键 词：脑缺血 电针 学习记忆 IL-1Β TNF-α 

分 类 号：R285.5[医药卫生—中药学]