新生大鼠缺氧缺血性脑损伤后脑组织中OMgp的表达  

The expression of OMgp in brain tissue of newborn rats after hypoxic-ischemic brain damage

作　　者：郭江波[1] 金冬梅[1] 

机构地区：[1]哈尔滨医科大学附属第一医院新生儿科,150000

出　　处：《中国新生儿科杂志》2015年第6期454-458,共5页Chinese Journal of Neonatology

摘　　要：目的探讨轴突生长抑制因子少突胶质细胞髓鞘糖蛋白(OMgp)在新生大鼠缺氧缺血性脑损伤(HIBD)后海马区的表达及意义。方法将128只7日龄SD大鼠随机分为HIBD组和对照组各64只。建立HIBD模型,取HIBD后1、3、7天和14天为观察点,采用实时荧光定量聚合酶链反应(RT-PCR)技术和免疫组织化学技术,检测各时间点OMgp在海马区的表达情况(n=8)。结果 RT-PCR结果显示,OMgp mRNA表达从HIBD后1天开始升高,7天达高峰,14天降低,并且HIBD组各个时间点OMgp mRNA表达量均高于对照组[1天:(1.54±0.12)比(1.11±0.19),3天:(2.56±0.14)比(1.14±0.08),7天:(2.95±0.11)比(1.43±0.17),14天:(2.53±0.11)比(1.08±0.08),P<0.05]。免疫组织化学结果显示,HIBD组与对照组比较,海马区OMgp蛋白表达在HIBD后1天开始升高,7天达高峰,14天表达降低,各时间点OMgp蛋白表达量均高于对照组[1天:(33.69±0.75)比(15.67±1.18),3天:(40.89±1.20)比(30.81±2.56),7天:(60.55±1.72)比(48.53±0.65),14天:(51.31±2.06)比(17.40±0.57),P<0.05]。结论 OMgp蛋白可能在未成熟中枢神经系统的发育及损伤中起重要作用。Objective To study the expression of axon growth inhibitory factor oligodendrocyte myelin glycoprotein （OMgp） in the hippocampus of newborn rats after hypoxic-ischemic brain damage （HIBD）, and to explore its significance. Methods Total of 128 SD （7-day-old） newborn rats were randomly assigned into HIBD group （n = 64） and control group （n = 64）. 1, 3, 7 and 14 days after HIBD were observation points in time. RT-PCR and immunohistochemistry were performed to detect the expression of OMgp in the hippocampus at different time point （ n = 8 ）. Results RT-PCR results indicated that the expression of OMgp mRNA increased from 1 d after HIBD, reached a peak at 7 d after HIBD, and decreased at 14 d after HIBD. The expression levels of OMgp mRNA at each time point in HIBD group was higher than that in control group [ 1 d ： （ 1.54 ± 0. 12） vs. （ 1.11± 0. 19）, 3 d ： （2. 56 ±0.14） vs. （1.14±0.08）, 7 d： （2.95 ±0.11） vs. （1.43 ±0.17）, 14 d： （2.53±0.11） vs. （ 1.08 ± 0. 08 ） , P 〈 0.05 ]. The same trend was seen in the expression of OMgp protein as well. Immunohistochemistry results showed that the expression of OMgp protein also kept increasing from 1 dafter HIBD a peak at 7 d after HIBD, and decreased at 14 d after HIBD. At the same time, the expression of OMgp protein in HIBD group at each time point was also higher than that in control group [ 1 d ： （33.69 ±0.75） vs. （15.67±1.18）, 3 d： （40.89±1.20） vs. （30.81 ±2.56）, 7 d： （60.55±1.72） vs. （48.53±0.65）, 14d： （51.31 ±2.06） vs. （17.40±0.57）, P〈0.05]. Conclusions OMgp protein may play an important role in the development and damage of the central nervous system in immature.

关 键 词：缺氧缺血 脑 少突胶质细胞髓鞘糖蛋白 实时聚合酶链反应 免疫组织化学 大鼠 

分 类 号：R722.1[医药卫生—儿科]