高吸水性树脂协同高强度聚焦超声对细粒棘球绦蚴原头节细胞酶活性的影响  

Effect of cell activity of protoscolices by super absorbent resin in coordination with high intensity focused ultrasound

作　　者：张静[1,2] 王梦莹[2] 叶彬[1,2] 赵毅峰[3] 韩秀敏[4] 李发琪 王琦 

机构地区：[1]重庆医科大学病原生物学教研室,重庆400016 [2]重庆医科大学分子与肿瘤研究中心,重庆400016 [3]青海大学医学院附属第一医院,西宁810001 [4]青海省地方病预防控制所,西宁811602 [5]省部共建超声医学工程国家重点实验室,重庆400016

出　　处：《中国人兽共患病学报》2015年第11期987-993,共7页Chinese Journal of Zoonoses

基　　金：国家自然科学基金(No.30972567);重庆市科学技术委员会(cstc2012jjA0032)资助~~

摘　　要：目的为探索高吸水性树脂(Super Absorbent Resin,SAR)协同高强度聚焦超声(high intensity focused ultrasound,HIFU)对离体细粒棘球绦蚴原头节酶活性的影响。方法将离体原头节悬液(每mL含有2 000个原头节),分为4组:I组(仅原头节悬液)、Ⅱ组:仅SAR组(0.01gSAR)、III组(单纯HIFU照射)、IV组(HIFU照射+0.01gSAR)、Ⅴ组(HIFU照射+0.1gSAR)。以声功率为100w的高强度聚焦超声波辐照30s,辐照后悬液涂片进行台盼兰及经冰冻切片酶组织化学染色,观察原头节形态学改变,并用半定量方法检测原头节内细胞的葡萄糖-6-磷酸酶和琥珀酸脱氢酶的活性。结果显示当超声剂量一定时,原头节的形态改变与SAR剂量有正效应关系,SAR量越大,其破坏程度越高。正常原头节透亮、外形完整,各实验组原头节深染或结构崩解。单纯HIFU照射组在作用60s时,原头节死亡率为80.1%;HIFU结合不同量SAR两组原头节在HIFU作用10s时,其死亡率分别为87.82%和89.1%。加入SAR后再经过HIFU照射各组原头节内细胞葡萄糖-6-磷酸酶和琥珀酸脱氢酶活性降低,明显低于单纯超声组(P<0.05),阴性对照组无酶反应物产。结论 SAR能增加HIFU辐照对原头节的急性杀伤作用,使原头节内细胞的葡萄糖-6-磷酸酶活性和琥珀酸脱氢酶活性均显著低降低,从而影响其活力。We investigated the effects of a combination of high intensity focused ultrasound （HIFU） and super absorbent resin on the cell activities of Echinococcus granulosus protoscoliees with enzyme histoehemical technique in vitro. The suspen- sion with 2 000 protoscoleces/ML from the hydatid cysts in sample sheep liver was divided into groups： group I （blank control） was without HIFU treatment and SAR in the suspension, group II was only SAR, group III was treated with HIFU only, group IV was treated with HIFU and 0.01 g SAR, and group~ was treated with HIFU and 0. lg SAR. Then, all these groups were exposed to the suspension of HIFU at a dose of 100 W acoustic powers for 30 seconds. The HIFU treated protoscolices were smeared onto glass slides for enzymatic activities localization. The protoscolex morphological changes and related enzyme activities in the HIFU treated protoscolices were determinated by the histochemical staining methods. Results showed the morphologieal changes of protoseoleees, the effect relationship was with dose of SAR, SAR was larger and the extent of damage was higher. There were clear and intact form in the nomal group, however, the protoscoleees showed stained and disintegrated in the tested groups. The death rate in the duratioh of single HIFU group was 80.1%, they were 87.82 %and 89.1% respectively in the HIFU combined SAR groups. In the groups of the combination of HIFU and SAR, the main en- zyme color of protoscoleces in shallow or disappeared, which suggested the treatment had an influence on enzyme activities of protoscolices. In conclusion, SAR could increase HIFU irradiation on the acute cytotoxicity and has certain delayed effect on protoscoleces, and the effect of HIFU on G-6-P and SDH in E. granulosus protoscolices was significantly low.

关 键 词：细粒棘球绦蚴原头节 高吸水性树脂 高强度聚焦超声 三磷酸腺苷酶 葡萄糖-6-磷酸酶 琥珀酸脱氢酶 

分 类 号：R383[医药卫生—医学寄生虫学]