高效毛细管电泳法鉴别土茯苓及其混伪品  

Distinguishing Smilax glabra and Its Six Falsified Specimens by High Performance Capillary Electrophoresis

作　　者：李玉琪[1] 李玉莲[1] 张文敏[1] 曾平[1] 杨自更[1] 姜维[1] 

机构地区：[1]中国人民解放军兰州军区乌鲁木齐总医院,新疆乌鲁木齐830000

出　　处：《时珍国医国药》2002年第8期469-470,共2页Lishizhen Medicine and Materia Medica Research

基　　金：兰州军区医药卫生科研项目 (项目编号 :L XH99- 2 0 )

摘　　要：目的 :利用高效毛细管电泳法鉴别土茯苓及其 6种常见混伪品。方法 :未涂层石英毛细管 5 0 μm× 5 0 cm,有效长度45 .5 cm,混合缓冲液为 5 0 mmol·L- 1 SDS- 30 mmol·L- 1硼酸 (p H8.3)、运行电压 2 0 k V,检测波长 2 10 nm。结果 :在该分离条件下 ,10 min可获得土茯苓及其 6种混伪品的特征性电泳图谱 ,通过电泳图谱可以很容易鉴别之。结论 :该方法简便、快速、准确 ,可作为中药土茯苓的质量控制方法。Objective: To develop a method for distinguishing Smilax glabra Roxb. from its six falsified plants of common occurrence by high performance capillary electrophoresis. Methods: Using a capillary (375 μmOD×50 μmID; 50 cm×45.5 cm) and a power supply set at 20 kV, with buffer containing 50 mmol·L -1 SDS-30 mmol·L -1 boric acid and a UV detector at 210 nm. Results: The characteristic electrophotograms of Smilax glabra and its six falsified plants were obtained under the above conditions in 10 min, they are easily distinguished by their electrophotograms. Conclusion: This method could serve as an easy and precise method for the quality control of Smilax glabra.

关 键 词：高效毛细管电泳法 土茯苓 赤土茯苓苷 药材鉴定 

分 类 号：R282.5[医药卫生—中药学]