HPLC同时测定麦冬中5个皂苷成分的含量  

HPLC simultaneous determination of contents of 5 saponin constituents in Ophiopogonis Radix

作　　者：吴发明[1] 蔡晓洋 王盼[1] 包小红 李敏[1] 周娟 

机构地区：[1]成都中医药大学中药材标准化教育部重点实验室,中药资源系统研究与开发利用国家重点实验室,四川成都611137 [2]四川省食品药品检验检测院,四川成都611000

出　　处：《中国中药杂志》2015年第20期4022-4025,共4页China Journal of Chinese Materia Medica

基　　金：四川省中医药管理局重点项目(2014F038);科技惠民计划项目(2013GS510102);十二五“中药质量安全检测和风险控制技术平台”(2014ZX09304307-002);四川省科技计划项目(2015SZ0033)

摘　　要：建立测定麦冬药材中麦冬皂苷D,麦冬皂苷D＇,ophiopojaponin C,去乙酰基ophiopojaponin A,3-O-α-L-鼠李糖-（1→2）-β-葡萄糖麦冬苷元5种成分的HPLC方法。采用HPLC-ELSD方法,色谱柱为Kromasil 100-5C18柱（4.6 mm×250 mm,5μm）;流动相为乙腈（A）-水（B）溶液,梯度洗脱0~45 min,35%~55%A,体积流量1.0 m L·min^-1;柱温35℃,漂移管温度100℃,气体流速3.0 L·min^-1;进样量15μL。结果表明5种成分均达到基线分离,各成分均有较宽的线性范围和良好的线性关系（r〉0.999）;回收率在95.75%~103.1%。建立的麦冬皂苷含量测定方法准确灵敏、重复性好,可应用于麦冬药材质量评价中。This research is to establish an HPLC method for the simultaneous determination of ophiopogonin D,ophiopogonin D＇,ophiopogonin C,deacetylophiopojaponin A and ophiogenin-3-O-α-L-rhamnosyl-（ 1→2）-β-D-glucoside in Ophiopogonis Radix. HPLCELSD analysis was performed on a Kromasil 100-5 C18column（ 4. 6 mm × 250 mm,5 μm）,with the mobile phase of acetonitrile（ A）-water（ B） in gradient elution mode（ 0-45 min,35%-55% A）,at a flow rate of 1 m L·min^-1. The column temperature was 35 ℃and the drift tube temperature was 100 ℃ in a gas flow rate of 3. 0 L·min^-1. The result showed that baseline of all the 5 constituents was well separated,and every constituent had wide linearity rangeand good linear relation（ r 0. 999）. The recovery rate was between95. 75% and 103. 1%. The new established method for simultaneous determination of saponin constituents in Ophiopogonis Radix was sensitive and has good,repeatability. It could be applied toquality evaluation of Ophiopogonis Radix.

关 键 词：麦冬 皂苷 HPLC 含量 

分 类 号：R284[医药卫生—中药学] O657.72[医药卫生—中医学]