基于液相蛋白芯片的3种中药有效组分配伍的抗炎作用研究  

Effects of combination of glycyrrhizin acid,ligustrazine and puerarin on LPS-induced cytokines expression in macrophage

作　　者：刘钊[1] 钟菊迎[1] 高尔宁 杨鸿[1] 

机构地区：[1]中国中医科学院医学实验中心,北京100700

出　　处：《中国中药杂志》2015年第20期4068-4074,共7页China Journal of Chinese Materia Medica

基　　金：中国中医科学院自主选题项目(ZZ2012001;ZZ2015008)

摘　　要：以3种中药有效组分配伍的抗炎活性为研究对象,基于液相蛋白芯片的高通量同步23种炎症因子检测技术,采用均匀设计结合综合权重法,按照均匀试验设计-药效试验-数学建模及模型验证-综合权重法药效评价程序,进行了甘草酸、川芎嗪和葛根素3种中药有效组分不同配伍剂量抑制LPS诱导RAW264.7细胞表达多种炎症细胞因子的研究。该实验采用均匀设计表U9(93)进行甘草酸、川芎嗪和葛根素3种有效组分剂量配伍设计;采用液相蛋白芯片技术检测3组分不同配伍组对脂多糖(LPS)诱导小鼠巨噬细胞RAW264.7炎症模型表达的23种细胞因子水平的影响;应用中药组分优化软件,采用改进最小角回归算法分析配伍3组分浓度和细胞因子抑制率间的量效关系,得到回归方程;结合综合权重法,获得甘草酸-川芎嗪-葛根素3种有效组分最优综合药效的最佳剂量组合为25∶2∶13,并对优化配伍进行实验验证;验证实验结果与预测趋势一致,说明该研究所建立的数学模型对该实验的预测非常准确。实验结果体现了甘草酸、川芎嗪和葛根素配伍多靶点、多方面、多层次的整体疗效的优势和特点,获得了具有较强抗炎活性的中药组分配伍,为后续药物开发提供有力依据。To study the anti-inflammatory activity of glycyrrhizin acid,ligustrazine and puerarin. In the study,the liquichip-based high-throughput synchronous detection technique for 23 inflammatory factors,uniform design,comprehensive weight method were adopted to study the effect of different combined administration of glycyrrhizin acid,ligustrazine and puerarin in inhibiting the expression of lipopolysaccharide（ LPS）-induced RAW264. 7 cells and multiple inflammatory cytokines. In the study,the uniform design table U9（ 93） was adopted to design doses of glycyrrhizin acid,ligustrazine and puerarin. The liquichip technique was used to detect the effect of different combined administration of glycyrrhizin acid,ligustrazine and puerarin on the 23 cytokines expressed in LPS-induced mouse macrophage RAW264. 7 inflammation model. The traditional Chinese medicine component optimization software and the improved least angle regression algorithm were used to analyze the dose-effect relationship among the three components and the cytokine inhibition rate and produce the regression equation. The comprehensive weight method was applied to get the optimal dose ratio of glycyrrhizic acid,ligustrazine and puerarin with highest efficacy of 25∶ 2∶ 13 and verify the optimal dose ratio. The verification results were consistent with the prediction trend,indicating the accuracy of the mathematical model for predicting the experiment. The experimental results showed the multi-target and multi-level efficacies of glycyrrhizic acid,ligustrazine and puerarin and the high anti-inflammatory activity of their combined administration,which provides powerful basis for subsequent drug development.

关 键 词：中药有效组分配伍 均匀设计 综合权重法 抗炎 

分 类 号：R285.5[医药卫生—中药学]