面向现场快速检测的微型化核酸扩增系统设计  

Design of miniaturized nucleic acid amplification system for spot rapid detection

作　　者：吴金龙[1] 杜耀华[1] 陈锋[1] 乔龙学[1] 韦婧[1] 李辰宇[1] 吴太虎[1] 

机构地区：[1]军事医学科学院卫生装备研究所,天津300161

出　　处：《军事医学》2015年第10期731-735,共5页Military Medical Sciences

基　　金：国家科技重大专项资助项目(2012ZX10004801)

摘　　要：目的设计面向现场快速检测的微型化核酸扩增系统。方法依据气浴式核酸扩增系统工作原理和传热特性,设计以颗粒堆积多孔介质均匀空气温度的微型化扩增系统,通过热力学仿真和温度循环实验验证其可行性。结果利用颗粒堆积多孔介质可有效均匀核酸扩增系统空气温度至0.8℃。微型核酸扩增系统体积参数为80 mm×40 mm×20 mm(长×高×宽),平均升温速率10℃/s,降温速率5℃/s。与标准PCR仪相比微型化核酸扩增系统扩增效果良好,满足设计要求。结论微型化核酸扩增系统反应速度快,便于携带,适用于现场对未知样品的核酸检测。Objective To present a miniaturized nucleic acid amplification system for spot rapid detection. Methods A miniaturized nucleic acid amplification system with structured packed porous media of particles to uniform the air temperature was designed according to the working principle and heat transfer characteristics of an air-heated nucleic acid amplification system. Thermodynamic simulation and temperature cycling test were carried on to verify the feasibility of the system. Results The structured packed porous media of particles worked well in uniforming the air temperature of the system and the temperature uniformity could reach 0.8~C. The miniaturized nucleic acid amplification system with a volume parameter of 80 mm x 40 mm x 20 ram（ length x height x width） was portable. The average rate of heating was 10~C/s while the average rate of cooling was 5~C/s. Compared with standard PCR instrument, the miniaturized nucleic acid amplification system performed well in the process of amplification and met the requirements of preliminary design. Conclusion The miniaturized nucleic acid amplification system with a rapid reaction velocity and portable volume could be applied to nucleic acid detection of unknown samples on the spot.

关 键 词：生物战剂 现场快速检测 微型化 核酸扩增系统 聚合酶链反应 基因组 

分 类 号：R440[医药卫生—诊断学]