不同氟浓度对大鼠原代软骨细胞Ⅱ型胶原表达的影响  

Effects of ColⅡexpression in different concentrations of fluoride in rat primary chondrocytes

作　　者：朱志坚[1] 于燕妮[1] 陶欣[1] 

机构地区：[1]贵阳医学院病理学教研室,贵州贵阳550004

出　　处：《贵州医药》2015年第7期577-579,F0003,共4页Guizhou Medical Journal

基　　金：国家自然科学基金(81260419);教育部博士点基金[博导类(20125215110001)]

摘　　要：目的观察不同氟浓度对大鼠原代软骨细胞Ⅱ型胶原(ColⅡ)表达的影响,探明ColⅡ在染氟软骨细胞的表达意义。方法取1周龄健康SD大鼠,用机械-酶消化法分离膝关节软骨细胞,经甲苯胺蓝鉴定后,取第三代细胞将实验分为对照组和染氟组(5mg/L、10mg/L、20mg/L、40mg/L)五组,培养3d后,经MTT检测各组细胞活力,并采用Western blot、免疫组化检测各组细胞ColⅡ蛋白表达。结果NaF培养3d后,细胞活力在5mg/L、10mg/L组较正常组高,在40mg/L组较正常组低,ColⅡ蛋白的表达在10mg/L组较正常组高,在40mg/L组较正常组低,差异有有统计学意义(P<0.05)。结论低浓度的氟对大鼠原代软骨细胞具有促进增殖作用,而高浓度具有抑制增殖作用,其机制可能与ColⅡ的分泌有关。Objective To investigate the effects of ColⅡ expression in different concentrations of fluoride in rat primary chondrocytes,and explore the significance expression of ColⅡ in chondrocytes with fluorine.Method The original generation of cartilage cells was obtained by mechanical-enzyme digestion method.The primary chondrocytes was identified by toluidine blue.After identified,the cell were divided into control group and NaF（5 mg/L,10mg/L,20mg/L,40mg/L）groups.After training 3d,the cell viability was determined by MTT in each group,the expressions of protein of ColⅡ in each group were detected using western blot（WB）and immunohistochemistry.Result NaF training after 3d,the cell vitality in 5and 10 mg/L groups were higher compared with normal group,and the 40mg/L group was lower than normal group,the ColⅡ protein expression in 10mg/L group was higher than normal group,and in 40mg/L group was lower than normal group,and there were statistical significantly differences（P〈0.05）.Conclusion Low concentration of fluoride can promote on the original generation cartilage cell proliferation in rats,and the high concentration could inhibit proliferation.The mechanism may be related to secretion of ColⅡ.

关 键 词：原代软骨细胞 氟化钠 Ⅱ型胶原 细胞活力 

分 类 号：R599.1[医药卫生—内科学] R361.3[医药卫生—临床医学]