人胃癌细胞系MKN-45中二甲双胍对顺铂的拮抗作用  

Preliminary study on the antagonistic effect of metformin on cisplatinin human gastric cancer MKN-45 cellline

作　　者：王晓东[1] 李红涛[2] 杨涛[2] 姚旻[2] 朱予津[2] 

机构地区：[1]武警天津总队医院药剂科,300162 [2]武警天津总队医院内二科,300162

出　　处：《武警医学》2015年第10期1015-1020,共6页Medical Journal of the Chinese People's Armed Police Force

摘　　要：目的初步研究二甲双胍(metformin,Met)和Met联合顺铂(cisplatin,Cisp)对人胃癌MKN-45细胞系的抗癌作用。方法应用不同浓度Met和Cisp单独或联合处理人胃癌MKN-45细胞系,MTT法分析细胞存活率,RT-PCR检测生存素、m TOR和Akt的mRNA表达水平,流式细胞仪检测细胞凋亡。结果单独使用Met可抑制增殖并诱导凋亡;Met联合Cisp时,可降低Cisp的抗增殖作用,且生存素、Akt和m TOR的mRNA表达明显增加(P<0.05)。Met对Cisp的拮抗作用可能是通过生存素、Akt和m TOR信号通路调节。结论 Met能抑制人胃癌MKN-45细胞系对Cisp化疗的敏感性,在患有2型糖尿病的胃癌患者中,应该慎重将Met与Cisp联合使用。Objective To evaluate the effect of Metformin and Metformin combined with Cisplatin on human gastric MKN -45 cell line.Methods Treated the human gastric cancer MKN-45 cell line withof the Metformin alone or Metformin combined withCis-platinby different concentrations ,thenanalysed the cell survival rate by MTT ,checked mRNA levels of survivin , mTOR, and Akt by re-al-time PCR, and assessed the Cell apoptosis by flow cytometry .Results Metformin alone could inhibit theproliferation and induce apoptosis, but it diminish anti-proliferative effects of theCisplatin when they were used in combination .When MKN-45 cells were trea-ted with Metformin and Cisplatin , the expression of survivin and mTOR were increased .The antagonistic effect of the Metformin on the Cisplatinmight be through survivin , Akt and mTOR signaling pathways .Conclusions Met can inhibit the chemotherapy sensitivity of CispinMKN-45 human gastric cancer cell line .For gastric cancer patients with Type 2 Diabeteswho treated with Cisp , Met should be carefully used.

关 键 词：二甲双胍 顺铂 胃癌 生存素 

分 类 号：R753.2[医药卫生—皮肤病学与性病学]