内蒙古自治区呼和浩特市荷斯坦犊牛全身性粟粒性结核病的诊断和病原鉴定  

DIAGNOSIS AND PATHOGEN IDENTIFICATION OF SYSTEMIC DISSEMINATED TUBERCULOSIS IN HOLSTEIN CALVES IN HUHHOT IN INNER MONGOLIA AUTONOMOUS REGION

作　　者：徐杰[1] 王金玲[2] 丁玉林[2] 周向梅[3] 

机构地区：[1]内蒙古财经大学资源与环境经济学院,呼和浩特010070 [2]农业部动物疾病临床诊疗技术重点实验室内蒙古农业大学兽医学院,呼和浩特010018 [3]中国农业大学动物医学院,北京100083

出　　处：《内蒙古农业大学学报（自然科学版）》2015年第4期11-15,共5页Journal of Inner Mongolia Agricultural University(Natural Science Edition)

基　　金：内蒙古农业大学博士启动基金(BJ07-51)

摘　　要：本研究采用尸体剖检、组织病理学和抗酸染色方法确诊了一起荷斯坦犊牛全身性粟粒性结核病疫情,此次疫情引起10头犊牛发病和死亡,随后提取下颌淋巴结组织DNA,采用多重PCR方法、测序和基因序列比对方法进行病原鉴定。结果显示发病牛全身淋巴结异常肿大,质硬,切面可见大面积的伴有大小不等钙化灶的干酪样坏死灶。在肝、肾、肺和脾的表面和切面以及在胸膜和小肠黏膜表面均可见多少不一的灰白色粟粒状的结核性肉芽肿或弥漫性干酪样坏死灶。通过组织病理学观察,在肿大的淋巴结、肝、肾、肺、脾、胸膜结节和小肠黏膜均可见特征性结核性肉芽肿,肉芽肿中央为干酪样坏死灶,坏死灶周围是由多核巨细胞和上皮样细胞构成的特殊肉芽组织以及淋巴细胞和结缔组织增生。通过抗酸染色,在淋巴结内可观察到大量细长红染的结核杆菌。多重PCR和PCR产物序列比对结果表明引起这起牛结核病疫情的病原为牛型结核分枝杆菌。近些年来,虽然牛结核在我国得到了一定程度的控制,但这起犊牛全身性粟粒性结核病疫情的发生也说明严重的牛结核病在内蒙古自治区呼和浩特市地区依然存在,一直威胁着养牛业的发展和人类的健康。Systemic disseminated tuberculosis resulting in death of 10 Holstein calves was confirmed by autopsy,histopathology and acid-fast staining in this study,and then the type of pathogen was ditermined by multiple PCR and sequence alignment of a PCR product. Results showed that most of lymph nodes were extremely enlarged and firm with large area of caseation. Many miliary tuberculous granulomas distributed on the surfaces and the cut surfaces of lung,liver,kidney,spleen,costal pleura and the mucosal surface of jejunum. Histopathologically,foci of caseation and dystrophic calcification in varying size were surrounded by multinucleated giant cells,epithelioid cells,lymphocytes and fibrous proliferation in the enlarged lymph nodes,lung,liver,kidney,spleen and jejunum. Masses of slender acid-fast bacilli were detected on Ziehl-Neelsen-stained sections of the enlarged lymph nodes. The results of multiple PCR and sequence alignment indicate that the pathogen of the systemic disseminated tuberculosis in Holstein calves was M. bovis. In recent years,although the bovine tuberculosis has been controlled to a certain extent in China,but this epidemic situation of disseminated tuberculosis also illustrates that serious bovine tuberculosis still exist in Hohhot city of Inner Mongolia and also threaten the development of cow industry and human health.

关 键 词：全身性粟粒性结核 荷斯坦犊牛 病理学 抗酸染色 病原鉴定 

分 类 号：S858.28[农业科学—临床兽医学]