ZS-SJZ结缕草与^(60)Co-γ辐射诱变新品系的抗寒性比较  

Comparison of cold resistance of ZS-SJZ zoysiagrass and its new strains induced by ^(60)Co-γ radiation

作　　者：宋华伟[1] 刘颖[1] 曹荣祥[1] 张巨明[1] 

机构地区：[1]华南农业大学林学与风景园林学院,广东广州510642

出　　处：《广东农业科学》2015年第20期39-44,共6页Guangdong Agricultural Sciences

基　　金：广东省科技计划项目(2012B020302002)

摘　　要：采用低温(5、10、20℃)处理ZS-SJZ结缕草(ZSS)及60Co-γ辐射诱变后筛选的2个新品系(ZSS-50-1和ZSS-60-2),通过测定叶片脯氨酸含量、相对电导率、可溶性总糖含量、超氧化物歧化酶(SOD)活性和丙二醛(MDA)含量等5个生理指标,同时观测草坪草颜色和盖度变化,比较3种材料之间抗寒性强弱。结果表明:随着胁迫温度的降低,3种材料的脯氨酸含量、叶片相对电导率、可溶性总糖含量、MDA含量和SOD活性均呈增大趋势,草坪颜色评分和盖度降低。在5℃低温胁迫下,新品系的脯氨酸含量、叶片相对电导率、可溶性总糖含量、MDA含量、颜色评分和盖度高于ZSS,而SOD活性低于ZSS。利用隶属函数标准差系数赋予权重法对3种材料的抗寒性进行综合评价,抗寒性由强到弱依次为ZSS-50-1>ZSS>ZSS-60-2。Study on cold resistance of ZS-SJZ zoysiagrass and its two new strains induced by 60Co-γ radiation, ZSS-50-1 and ZSS-60-2 was conducted under low temperature at 5℃, 10℃, 20℃ respectively. The cold resistance of the three zoysiagrasses was evaluated by measuring proline content, relative electrical conductivity, soluble total sugar content, superoxide dismutase （ SOD ） activity and malondialdehyde （ MDA ） content of leaves, and the turfgrass coverage and color were observed. The results showed that the proline content, leaf relative electrical conductivity, soluble sugar content, MDA content and SOD activity of the three zoysiagrasses showed an higher trend with the decrease of temperature stress, whereas the turfgrasses coverage and color ratings were reduced. Under 5 ℃ treatment, the proline content, relative electrical conductivity, soluble total sugar content and MDA content of the new turfgrass strains were greater than those of ZSS zoysiagrass, while the SOD activity was lower than that of ZSS zoysiagrass. Using the method of subordinate function weights coefficient of standard deviation the cold resistance of the three zoysiagrasses was comprehensively evaluated, the order of cold resistance ability was ZSS-50-1 〉 ZSS 〉 ZSS-60-2.

关 键 词：ZS-SJZ结缕草 60CO-Γ辐射 新品系 隶属函数 抗寒性 

分 类 号：S688.4[农业科学—观赏园艺]