对照提取物法测定女贞子饮片中4种成分的含量  

Content Determination of 4 Ingredients in Ligustrum lucidum Decoration Piece by Reference Extract Method

作　　者：陶益[1] 张畅 李伟东[1] 陆兔林[1] 蔡宝昌[1] 

机构地区：[1]南京中医药大学中药炮制重点实验室,南京210046

出　　处：《中国药房》2015年第33期4685-4687,共3页China Pharmacy

基　　金：国家科技重大专项(No.2012ZX09304005);江苏省自然科学基金青年项目(No.BK20140963)

摘　　要：目的：建立对照提取物法测定女贞子饮片中4种成分含量的方法。方法：采用高效液相色谱法。色谱柱为XBridge C18,流动相为乙腈-0.1%甲酸（梯度洗脱）,流速为1.0 ml/min,检测波长为254 nm,柱温为30℃,进样量为10μl。结果：新女贞苷、毛蕊花糖苷、女贞苷、特女贞苷检测质量浓度线性范围分别为0.037~0.598、0.006~0.101、0.189~3.023、0.314~5.027 mg（r均≥0.9990）;精密度、重复性、稳定性试验的RSD≤3.80%;加样回收率分别为98.46%~104.83%（RSD=2.43）、95.55%~104.57%（RSD=3.63）、100.09%~104.39%（RSD=1.45）、98.84%~104.97%（RSD=2.02）,n均为6。对照提取物法与单一对照品测定法所得结果差异无统计学意义（P〉0.05）。结论：该方法操作简便、重复性好,可用于女贞子饮片中4种成分含量的测定。OBJECTIVE：To establish a method for the contents determination of 4 ingredients in Ligustrum lucidum decoration piece by reference extract method. METHODS：HPLC was performed on the column of XBridge C18 with mobile phase of acetonitrile-0.1% formic acid（gradient elution） at flow rate of 1.0 ml/min,detection wavelength was 254 nm,column temperature was30 ℃ and volume injection was 10 μl. RESULTS：The linear range was 0.037-0.598 mg for neonuezhenide,0.006-0.101 mg for acteoside,0.189-3.023 mg for nuezhenide and 0.314-5.027 mg for specnuezhenide（r≥0.999 0）;RSDs of precision,reproducibility and stability tests were no more than 3.8%;recoveries were 98.46%-104.83%（RSD=2.43,n=6）,95.55%-104.57%（RSD=3.63,n=6）,100.09%-104.39%（RSD=1.45,n=6）and 98.84%-104.97%（RSD=2.02,n=6）. CONCLUSIONS：The method is simple,good reproducibility,and can be used for the contents determination of 4 ingredients in L. lucidum decoration piece.

关 键 词：女贞子 对照提取物法 高效液相色谱法 

分 类 号：R927.2[医药卫生—药学]