HPCE法测定水曲柳叶中荷包花苷A的含量  

Content Determination of Calceolarioside A in the Leaves of Fraxinus mandschurica by HPCE

作　　者：陈玉娟[1] 王巍[1] 李成玉[1] 张佳佳[1] 杨业[1] 李步步 

机构地区：[1]长春理工大学生命科学技术学院,长春130022

出　　处：《中国药房》2015年第33期4709-4710,共2页China Pharmacy

基　　金：吉林省科技重点科技攻关项目(No.20140204052YY)

摘　　要：目的：建立测定水曲柳叶中荷包花苷A含量的方法。方法：采用高效毛细管电泳法。毛细管为未涂层熔融石英毛细管,缓冲液为硼酸盐缓冲盐（20 mmol/L,p H 9.3）,分离电压为20 KV,采用压力进样（进样压力为50 bar,进样时间为3 s）,检测波长为350 nm,柱温为30℃。结果：荷包花苷A检测质量浓度线性范围为50~250μg/ml（r=0.999 7）;精密度、稳定性、重复性的RSD〈3%,加样回收率为97.48%~102.08%,RSD为1.12%（n=6）。结论：该方法操作简便、重复性好,可用于测定水曲柳叶中荷包花苷A的含量。OBJECTIVE：To establish a method for the content determination of calceolarioside A in the Leaves of Fraxinus mandschurica. METHODS：HPCE was performed on the capillary of uncoated fused silica capillary,buffer solution of borate buffered saline（20 mmol/L,p H 9.3）,separation voltage of 20 KV by pressure injection（injection pressure was 50 bar with 3 s）,detection wavelength was 350 nm,and column temperature was 30 ℃. RESULTS：The linear range of calceolarioside A was50-250 μg/ml（r=0.999 7）;RSDs of precision,stability and reproducibility tests were less than 3%,recovery was 97.48%-102.08%（RSD=1.12,n=6）. CONCLUSIONS：The method is simple,good reproducibility,and can be used for the content determination of calceolarioside A in the Leaves of F. mandschurica.

关 键 词：水曲柳 叶 荷包花苷A 高效毛细管电泳法 

分 类 号：R917[医药卫生—药物分析学]