RhoA相关信号通路的表达对COPD大鼠气道重塑的影响  

Effects of RhoA related signaling pathways on airway remodeling of COPD rats

作　　者：徐新毅[1] 刘友琴[2] 徐丹[3] 杨义[3] 

机构地区：[1]贵阳中医学院第二附属医院,贵州贵阳550003 [2]贵州省人民政府机关门诊部,贵州贵阳550004 [3]贵阳中医学院,贵州贵阳550002

出　　处：《贵州医药》2015年第10期880-882,共3页Guizhou Medical Journal

摘　　要：目的观察正常对照组大鼠、COPD模型组大鼠及地塞米松治疗组大鼠肺组织中RhoA mRNA及蛋白含量的变化,探讨RhoA信号通路在COPD大鼠气道重塑中的作用。方法将60只雄性大鼠随机分为3组,每组各20只,采用烟熏加脂多糖的方法建立COPD大鼠模型,造模成功后,用免疫组化法测定各组大鼠肺组织RhoA蛋白的表达量,Real-time PCR法检测各组大鼠RhoA mRNA含量。结果与正常对照组比较,COPD模型组大鼠RhoA mRNA的表达增高(P<0.05),与COPD模型组比较,地塞米松治疗组RhoA mRNA的表达降低(P<0.05);免疫评分结果显示:与正常对照组比较,COPD模型组大鼠RhoA蛋白阳性表达率增高(P<0.05),地塞米松组RhoA蛋白阳性表达率降低(P<0.05)。结论 RhoA信号通路在大鼠气道重塑这一病理变化中起一定作用,地塞米松对COPD大鼠的气道重塑具有抑制作用。Objective To observe the level changes of RhoA-mRNA and protein content in the rats＇ lung tissue which were randomly divided into three groups that were COPD group,NS group,Dexamethasone treatment group,exploring the effect of RhoA-mediated signal pathway in airway remodeling in COPD rats.Method 60 SD male rats were randomly divided into NS group,COPD group,Dexamethasone treatment group,and 20 in each group.The COPD rat model was built with compound factors that cigarette smoke plus intratracheal instillation of lipopolysaccharide（LPS）.After duplicating the mold,animals were put to death and collected samples.The expression of protein content in the rats＇ lung tissue was detected in both groups by immunohistochemistry.The expression of mRNA was detected by RT-qPCR.Result Compared with NS group,the expression of RhoA-mRNA in COPD group shown increasing trend,and there were statistical significantly differences（P〈0.05）.Compared with NS group,the expression of RhoA-mRNA in Dexamethasone treatment group shown a decreasing trend（P〈0.05）.Compared with NS group,Immunohistochemical results shown that the protein positive rate of RhoA-mRNA was significantly increased in COPD group（P〈0.05）,was significantly decreased in COPD group（P〈0.05）.Conclusion RhoA-mediated signal pathway may play a role in airway remodeling in COPD rats.Dexamethasone is effective in reducing airway remodeling.

关 键 词：RhoA/rock信号通路 慢性阻塞性肺疾病 气道重塑 地塞米松 

分 类 号：R563.9[医药卫生—呼吸系统]