蕨麻正丁醇部位下调缺氧心肌细胞CaV1.2及Ryanodine受体表达  

Down-regulated expressions of Cav1.2 and ryanodine receptor mRNAs by n-butanol fraction of Potentilla anserine L. in myocardial cells exposed to hypoxia

作　　者：苏畅[1] 王鲁君[2] 张永亮 李灵芝[1,3] 李功甫 刘洋[1] 

机构地区：[1]武警后勤学院救援医学系,天津300309 [2]武警总医院药剂科,北京100039 [3]天津市职业与环境危害防制重点实验室,天津300309

出　　处：《武警后勤学院学报（医学版）》2015年第10期770-774,共5页Journal of Logistics University of PAP（Medical Sciences）

基　　金：国家自然科学基金项目(81471823);天津市重点项目(12JCZDJC34700);武警后勤学院创新团队科学基金资助项目(WHTO201303)

摘　　要：【目的】研究蕨麻正丁醇部位对大鼠心肌细胞缺氧所致钙超载的抑制作用,并通过研究其对心肌细胞L-型钙通道(Ltype calcium channel,Cav1.2)及肌浆网兰尼定受体(Ryanodine receptor,Ry R)表达的影响,探讨其可能的机制。【方法】建立原代心肌细胞缺氧模型,设正常对照组、缺氧损伤组、维拉帕米组(5μmol/L)及蕨麻正丁醇部位高、中、低浓度组(250.0、62.5、15.6μg/ml)。采用荧光分光光度法检测各组心肌细胞内钙离子变化,RT-PCR法检测心肌细胞Cav1.2和Ry R m RNA表达水平。【结果】缺氧损伤组心肌细胞内游离钙离子的浓度较正常对照组明显增加(P<0.01),Cav1.2 m RNA、Ry R m RNA表达明显增强(P<0.01),维拉帕米组和蕨麻正丁醇部位高、中、低浓度组心肌细胞内游离钙离子的浓度较缺氧损伤组明显降低,Cav1.2 m RNA(P<0.01或P<0.05)和Ry R m RNA(P<0.01)表达明显降低。【结论】蕨麻正丁醇部位可明显抑制缺氧所致心肌细胞钙超载,抑制L-型钙通道和Ry R的表达。提示蕨麻正丁醇部位抑制心肌细胞内钙超载的作用可能与其抑制L型钙通道有关。[Objective] To study the inhibiting effect of the n-butanol fraction of Potentilla anserine L. on hypoxia-induced calcium overload in rat myocardiocytes and explore its underlying mechanisms by its influence on L-type calcium channel （Carl.2） and ryanodine receptor （RyR） expression. [ Methods ] Hypoxia models of primary myocardiocytes were established. There were 6 groups： control group, hypoxia injury model group, verapamil group（5μmol/L）, intervention group with n-butanol fraction of Potentilla anserine L. （250.0 μg/ml, 62.5 μg/ml and 15.6 μg/ml）. The calcium concentration in myocardial cells was determined with fluorospectro- photometry. The expression levels of RyR and Cavl.2 in myocardial cells were detected with RT-PCR method. [ Results] Compared with the control group, the dissociative calcium concentration of the hypoxia injury group increased obviously （P〈0.01）; the mRNA expression levels of Cavl.2 and RyR also increased statistically （P〈O.01）, the calcium concentration in verapamil group and all intervention groups decreased, and the expression levels of Cavl.2 mRNA （P〈0.05 or P〈0.01） and RyR mRNA （P〈0.01） reduced distinctly. [ Conclusion ] The n-Butanol fraction of Potentilla anserine L. can attenuate the calcium overload induced by hypoxia and down-regulate expressions of Cavl.2 mRNA and RyR mRNA. It indicates that the inhibiting effect of n-butanol fraction of Potentillaanserine L. on calcium overload in myocardial cells might be related to its down-regulation of L-type calcium channel expression.

关 键 词：心肌细胞 缺氧 钙超载 蕨麻 L型钙通道 兰尼定受体 

分 类 号：R285.5[医药卫生—中药学]