德都红花-7味散对肝纤维化组织中转化生长因子β1及smad蛋白通路的影响  

Influences of deduhonghua-7 powder on transforming growth factor beta and smad protein pathways in liver fibrosis tissue

作　　者：徐艳华[1] 巴图德力根[1] 韩志强[1] 山丹[2] 塔娜[2] 娜日苏[1] 安达[1] 

机构地区：[1]内蒙古民族大学附属医院蒙药临床药理研究所,内蒙古通辽028007 [2]内蒙古民族大学蒙医药学院,内蒙古通辽028000

出　　处：《中国临床药理学杂志》2015年第22期2246-2248,共3页The Chinese Journal of Clinical Pharmacology

基　　金：国家自然科学基金资助项目(81260574)

摘　　要：目的探讨德都红花-7味散治疗肝纤维化的作用及其与转化生长因子β1及smad蛋白通路的相关性。方法将肝纤维化大鼠随机分为A组、B组、C组,每组8只;同时,另取8只正常大鼠,作为D组。A组予以灌胃0.6 g·kg-1德都红花-7味散,每天1次;B组予以灌胃0.4 mg·kg-1秋水仙碱,每天1次;C组和D组同时予以蒸馏水,每天1次。4组均连续给药40 d。用弹力胶原纤维染色法观察肝组织纤维化程度。用酶联免疫法检测肝组织匀浆透明质酸、层黏连蛋白含量。用免疫组织化学法检测肝smad2、smad3、smad6、smad7、转化生长因子β1蛋白表达变化。结果 C组胶原纤维较D组增多,A组和B组胶原纤维较C组减少。C组透明质酸、层黏连蛋白含量较D组升高(P<0.05),A组和B组透明质酸、层黏连蛋白含量较C组降低(P<0.05)。C组转化生长因子β1、smad2、smad3蛋白表达较D组上调(P<0.05),A组和B组转化生长因子β1、smad2、smad3蛋白表达较C组下调(P<0.05)。C组smad6、smad7蛋白表达较D组下调(P<0.05),A组和B组smad6、smad7蛋白表达较C组上调(P<0.05)。结论德都红花-7味散通过调节转化生长因子/smad蛋白通路,从而阻断转化生长因子表达,抑制细胞外基质的合成,促进细胞外基质的降解,达到阻断或延缓肝纤维化发展的目的。Objective To observe the therapeutic effect of deduhonghua -7 powder on liver fibrosis and explore its relevance with transforming growth factor beta （TGF-β ） and smad protein pathways. Methods The liver fibrosis rat model were randomly divided to A group, B group, C group, D group, 8 rats for each. A group was administered 0. 6 g ·kg^-1 of deduhonghua - 7 powder, B group was administered 0. 4 mg ·kg^-1 of colchicines, C group and D group were administered dis- tilled water intragastrically 1 time per day for 40 days. The liver tissue was prepared by histopathology slides, than elastic and collagen fiber staining was acted to observe the degree of fibrosis. Enzyme - linked im- munosorbent assay （ELISA） was acted to detect the hyaluronic acid （HA） , laminin （LN） content of liver tissue homogenate. Immunohisto- chemistry detecting was acted to determine the changes of smad2, smad3, smad6, smadT, and TGF -β1 protein expressions. Results Compared with D group, the collagen fiber and contents of HA, LN of C group significantly increased （P 〈 0. 05 ）. Compared with C group, the collagen fiber and the contents of HA, LA of A group and B group significantly decreased （P 〈 0. 05 ）. The TGF - β1, smad2, smad3 protein expressions of C group were up - regulated compared with those of D group with significant differences （P 〈 0. 05 ）. The TGF - β1, smad2, smad3 protein expres- sions of A and B groups were down - regulated compared with those of C group, with significant differences （P 〈 0. 05 ）. The smad6, smad7 protein expressions of C group were down - regulated compared with those of D group, with significant differences （P 〈 0. 05 ）. The smad6, smad7 protein expressions of A and B groups were up - regulated com- pared with C group, with significant differences （P 〈 0. 05）. Conclusion Deduhonghua -7 powder can regulate the TGF - β/smad protein patheways and block the TGF - 15 expression, inhibit the synthesis of extracellular matrixc and degradation of extracellula

关 键 词：德都红花-7味散 肝纤维化 转化生长因子Β1 SMAD蛋白 

分 类 号：R657.3[医药卫生—外科学] R966[医药卫生—临床医学]