丙泊酚和氯胺酮对幼年大鼠认知及免疫功能的影响  

Effect of propofol and ketamine anesthesia on cognitive function and immune function in young rats

作　　者：刘德君[1] 董铁立[1] 

机构地区：[1]郑州大学第二附属医院麻醉科,450014

出　　处：《中华实验外科杂志》2015年第11期2751-2753,共3页Chinese Journal of Experimental Surgery

摘　　要：目的 观察丙泊酚和氯胺酮对幼年大鼠认知及免疫功能的影响.方法 80只14 d幼年大鼠随机分成4组：对照组、A组（氯胺酮组80 mg/kg）、B组（丙泊酚组80 mg/kg）、C组（氯胺酮和丙泊酚各80 mg/kg）.腹腔连续注射药物3d.检测幼年大鼠血清白细胞介素（IL）-2、IL-4、IL-10水平,测定大脑海马神经元凋亡水平及水迷宫实验测试幼年大鼠认知能力.结果 水迷宫测试：第1天,对照组潜伏期（112.6±25.1）s、B组（114.1±27.2）s及C组（108.9±23.8）s,水迷宫测试潜伏期逐渐降低;第3天,与对照组（53.1±11.5）s比较,A组（103.8±18.1）s潜伏期及穿越平台次数显著降低;4组大脑海马神经元凋亡率分别为（2.2±0.6）％、（15.1±7.3）％、（4.3±1.1）％、（10.6±2.4）％,A组神经元凋亡率显著增高.A、C组血清IL-4和IL-10水平均明显高于对照组[（0.4±0.1）、（0.8±0.3） μg/L].结论 丙泊酚能降低氯胺酮对幼年大鼠认知及免疫功能的影响.Objective To investigate the effects of propofol and ketamine on the cognitive function and immune function in young rats.Methods A total of 80 young rats were randomly divided into four groups： control group, ketamine group （experimental group A）, propofol group （experimental group B）, and ketamine and propofol group （experimental group C）.All rats were subjected to continuous injection for three days.The expression level of interleukin （IL）-2, IL-4 and IL-10 in serum, as well as the hippocampal neuronal apoptosis level was measured.The cognitive ability of rats was tested by water maze test.Results Water maze test showed that on the first day, the maze test latency in the control group （112.6 ± 25.1） s, the experimental group B （114.1 ± 27.2） s and the experimental group C （108.9 ± 23.8） s was decreased gradually.At 3rd day, the latency in the experimental group A （103.8 ± 18.1） s was significantly decreased, and the crossing table times were also reduced as compared with the control group （53.1 ± 1 1.5） s.Hippocampal neuron apoptosis rate in control group, experimental group A, experimental group B and experimental group C was （2.2 ±0.6）%, （15.1 ±7.3）% , （4.3 ± 1.1）% and （10.6 ± 2.4）% respectively.The apoptosis rate of hippocampal neurons in experimental group A was significantly increased.The levels of serum IL-4 and IL-10 levels in experimental group A, and experimental group C after anesthesia were significantly higher than in the control group [（0.4 ±0.1）, （0.8 ± 0.3） μg/L].Conclusion Propofol can reduce the effect of ketamine on the cognitive function and immune function in the young rats.

关 键 词：丙泊酚 氯胺酮 认知功能 免疫功能 

分 类 号：R720.597[医药卫生—急诊医学]