二氧化碳浓度对A549细胞活性和肺泡表面活性蛋白C表达的影响  

Effect of carbon dioxide concentration on the activity of A549 cells and the expression of pulmonary surfactant protein C

作　　者：彭丹晖[1] 黄英明[1] 老启芳[2] 覃韬[2] 谢显龙[2] 黄冰[2] 黎阳[2] 

机构地区：[1]广西医科大学附属肿瘤医院麻醉科,南宁530021 [2]广西医科大学附属肿瘤医院重症医学科,南宁530021

出　　处：《中华实验外科杂志》2015年第11期2761-2763,共3页Chinese Journal of Experimental Surgery

基　　金：广西医疗卫生重点科研课题资助项目（重2010078）

摘　　要：目的 观察CO2培养浓度对A549细胞活性,合成和分泌肺泡表面活性蛋白C（SP-C）的影响.方法 A549细胞按CO2培养浓度分为：A组（5％CO2）;B组（10％ CO2）;C组（18％ CO2）.各组在CO2培养24、36、48 h后,流式细胞仪检测细胞周期和凋亡率;反转录-聚合酶链反应（RT-PCR）检测SP-C mRNA表达;酶联免疫吸附法检测细胞培养液中SP-C浓度.结果 （1）与A组比较,B组G1峰和凋亡率在24、36、48 h,差异无统计学意义（P＞0.05）;C组与B组比在24h（68.0±6.2比55.0±3.8,P＜0.01）、36 h（71.0±2.0比63.0±1.3）出现G1峰升高（P＜0.05）,并于36 h（10.0±1.2比6.9±1.9）、48 h（12.8 ±2.6比7.3±2.0）凋亡增加（P＜0.05）.（2）3组SP-C mRNA表达在24h比较差异无统计学意义（P＞0.05）;与A组比较,B组在36 h（0.32±0.02比0.24±0.02）（P＜0.01）、48 h（0.24±0.03比0.16±0.03）SP-C mRNA表达升高（P＜0.05）,而C组均降低（0.17 ±0.04、0.10 ±0.02） （P ＜0.05）.（3） SP-C 3组浓度在24h比较差异无统计学意义（P＞0.05）;与A组比较,B组SP-C浓度在36 h（31.00±2.00比21.00±0.85）（P＜0.01）,48 h（42.00±1.02比26.00±0.97）升高（P＜0.05）;C组SP-C浓度在48 h（20.00±0.51比26.00±0.97）降低（P＜0.05）,24、36 h比较差异无统计学意义（P＞0.05）.结论 A549细胞活性和SP-C的表达受CO2培养浓度的影响;随着CO2浓度的升高,其作用由上调转为抑制.Objective To investigate the effects of CO2 concentration on the activity of A549 cell, the synthesis and secretion of puhmonary surfactant protein C （SP-C）.Methods A549 cells in CO2 culture were divided into three groups, group A （5% CO2）;B group （10% CO2）;group C （18% CO2）.After culture of 24, 36, 48 h.The cell cycle progression and apoptosis were measured by flow cytometric analysis, the expression of SP-C mRNA was measured by reverse transcriptase-polymerase chain reaction （RT-PCR）, and the SP-C concentration in the cell culture supernatants was measured by enzyme-linked immunosorbent assay method.Results （1） Compared with A group, B group＇ s G1 peak and the rate of apoptosis in 24, 36 and 48 h showed no significant difference （P 〉0.05）.In the C group, The G1 peak increase in 24h （68.0 ±6.2 vs.55.0 ±3.8） and 36 h （71.0 ±2.0 with 63.0 ± 1.3） （P 〈0.01;P 〈 0.05）, and the apoptosis rate increased in 36 h （10.0 ± 1.2 vs.6.9 ± 1.9） and 48 h （12.8 ± 2.6 with 7.3 ± 2.0） （P 〈 0.05）.（2） The SP-C mRNA expression of the three groups in 24 hours were no difference （P 〉 0.05）.According to A group, B group＇ s mRNA of SP-C increased in 36 h （0.32 ± 0.02 vs.0.24±0.02） （P〈0.01） and48 h （0.24±0.03 vs.0.16±0.03） （P〈0.05）;On the contrary,they are all drop in the C group （0.17 ±0.04, 0.10±0.02） （P〈0.05）.（3）The SP-C concentration of the three groups in 24 hours were no difference （P 〉 0.05）.According to A group, B group＇s concentration ofSP-C increased in 36 h （31.00 ±2.00 vs.21.00 ±0.85, P〈0.01） and 48 h （42.00 ± 1.02 vs.26.00 ± 0.97, P 〈 0.05）;Compared with A group, C group＇ s concentration of SP-C decreased in 48 h （20.00 ± 0.51 vs.26.00 ± 0.97, P 〈 0.05）, and 24 h and 36 h showed no significant difference （P 〉 0.05）.Conclusion The activity of A549 cells and the expression of SP-C were influenced by the CO2 concentration of culture.With the increasing of CO2 concentrati

关 键 词：A549 反转录-聚合酶链反应 肺泡表面活性蛋白C 

分 类 号：R852.82[医药卫生—航空、航天与航海医学]