腰动脉结扎后脊髓缺血神经损伤与修复的研究  

Spinal cord ischemia injury and rehabilitation after lumbar artery ligation

作　　者：傅超锋 庄源东 陈春美[1] 王春华[1] 赵伟[1] MD GOLAM KIBRIA 

机构地区：[1]福建医科大学附属协和医院神经外科福建省神经外科研究所,福州350001

出　　处：《中华实验外科杂志》2015年第11期2803-2806,共4页Chinese Journal of Experimental Surgery

摘　　要：目的 观察SD大鼠腰动脉结扎脊髓缺血损伤后Fas、含半胱氨酰天冬氨酸特异性蛋白酶-3（Caspase-3）、肿瘤坏死因子-α（TNF-α）、核因子-κB（NF-κB）、神经生长因子（NGF）、生长相关蛋白-43（GAP-43）及脑源性神经生长因子（BDNF）表达的变化,探讨SD大鼠脊髓缺血性损伤后自我修复的过程.方法 56只SD大鼠随机分为缺血组和假手术组.假手术组14只,缺血组42只,缺血组分别再随机分为缺血1、2、4、6、8、12和24h组,以假手术组相同阶段的脊髓为对照,采用神经功能评定法（BMS评分）评估各组行为学的变化,并采用免疫组织化学方法检测脊髓中Fas、Caspase-3、TNF-α、NF-κB、NGF、GAP-43及BDNF的表达变化.结果 缺血组SD实验大鼠BMS评分在缺血后立即出现显著降低[（0.58 ±0.66）分],至缺血后24h逐渐恢复至正常水平[（8.33±0.61）分],与假手术组比较差异无统计学意义（P＞0.05）,免疫组织化学染色结果显示Fas、Caspase-3、TNF-α、NF-κB表达的阳性细胞数在缺血后立即显著增多,其值分别为（45.50±4.18、47.50±3.27、53.50±7.71、57.83±4.79）,与假手术组比较差异均有统计学意义（P＜0.05）,除Fas外（7.50±1.87）,其余各个指标至缺血后24h逐渐恢复至正常水平,与假手术组比较差异均无统计学意义（P＞0.05）.GAP-43在缺血后阳性表达细胞数立即增多（6.33±1.21）,缺血后各组与假手术组比较差异均有统计学意义（P＜0.05）,NGF在缺血后8 h（37.33±1.37）阳性表达细胞数才开始增多,随后逐渐升高,缺血后8、12、24 h与假手术组比较均有统计学意义（P＜0.05）,BDNF阳性表达细胞数在缺血后1、2h（22.83±1.94、23.83±1.94）显著低于假手术组,于缺血后4 h（31.33±2.16）开始上升,随后逐渐升高,缺血后4、6、8、12、24 h与假手术组比较差异均有统计学意义（P＜0.05）.结论 SD大鼠全部腰动脉结扎致脊髓缺血性损伤后24 h可恢�Objective To investigate the change of cysteinyl aspartate-specific protease-3 （Caspase-3）, tumor necrosis factor-α （TNF-α）, nuclear factor-κB （NF-κB）, nerve growth factor （NGF）, growth associated protein 43 （GAP-43）, and brain derived neurotrophic factor （BDNF） expression after spinal cord ischemia injury in the SD rats by ligating lumbar arteries, and observe the process of self-repair after spinal cord ischemia injury in the SD rats.Methods Fifty-six SD rats were divide into ischemia group （n =42） and sham operation group （n =12） randomly.The SD rats in ischemia group were subdivided into 1,2, 4, 6, 8, 12 and 24 h subgroups.BMS score was adopted to assess the change of behavior.The expression of Fas, Caspase-3, TNF-α, NF-κB, NGF, GAP-43 and BDNF was detected by immunohistochemistry.Results In ischemia group, BMS score was obvious decrease immediately after ischemia injury （0.58 ±0.66） and return to normal level （8.33 ±0.61） 24 h after ischemia injury, there was no significant difference compared with control group （P 〉 0.05）.Immunohistochemical study showed obvious increase were found in Fas, Caspase-3, TNF-α, NF-κB activity in ischemia group, the value were （45.50 ±4.18, 47.50 ±3.27, 53.50 ±7.71,57.83 ±4.79） respectively,which had statistically difference compared with control group （P 〈 0.05）, and return to normal level 24 h after ischemia injury except Fas （7.50 ± 1.87）.GAP-43 positive cell number increased immediately after ischemia injury （6.33 ± 1.21） and had statistically difference compared with control group in each group （P 〈0.05）.The number of NGF positive cell were increase 8 h after ischemia injury （37.33 ± 1.37） and in 8, 12, 24 h after ischemia injury there had statistically difference compared with control group.BDNF activity cell number were significantly lower than control group 1 h and 2 h after ischemia injury （22.83 ± 1.94, 23.83 ± 1.94） and increased 4 h after ischemia injury （31.33 ±2.16�

关 键 词：脊髓缺血性损伤 炎症 修复 脱噬作用 

分 类 号：R651.15[医药卫生—外科学]