2013年-2014年东莞市麻疹监测结果分析  

Analysis of measles surveillance results in Dongguan during 2013-2014

作　　者：李艳芬[1] 黎景全[1] 袁达康[1] 杨华可[1] 陈永迪[1] 黄勇[1] 

机构地区：[1]东莞市疾病预防控制中心,广东东莞523129

出　　处：《中国卫生检验杂志》2015年第21期3731-3732,3736,共3页Chinese Journal of Health Laboratory Technology

摘　　要：目的分析2013年9月-2014年12月东莞市麻疹实验室监测结果,了解麻疹的流行状况和病毒基因型特征。方法采用ELISA法对血清标本进行麻疹Ig M抗体检测,采用荧光RT-PCR法对咽拭子或尿液标本进行麻疹病毒核酸检测,核酸阳性标本送至广东省疾病预防控制中心进行基因分型。结果收集血清标本723份,Ig M抗体阳性524份,阳性率为72.48%;收集522份咽拭子或尿液标本,核酸阳性412份,阳性率为78.93%。成功对168份标本进行基因分型,其中H1a基因型163份,A型2份,B3型、D9型、G3型各1份。抗体检测结果阴性及未进行抗体检测的126例病例中,检出39例麻疹核酸阳性。结论东莞市麻疹流行以基因型H1a为主,并存其他基因型的散发流行。其中D9基因型为广东省首次发现的基因型。应加强结合血清学检测和分子生物学技术,以提高麻疹病原学监测的检出率。Objective To analyze the measles surveillance results from September 2013 to December 2014,so as to understand the prevalence of measles in Dongguan and the virus genotype characteristics. Methods The Ig M antibody were detected by ELISA from the serum sample,and the throat swab or urine samples were detected by fluorescent RT- PCR for measles virus RNA,and the positive RNA samples were sent to Guangdong CDC to detect the genotyping. Results Total 723 serum samples were tested,there were 524 Ig M positive samples,and the positive rate was 72. 48%. 412 PCR positive samples were detested from the 522 throat swab or urine samples,the positive rate was 78. 93%. 168 samples had successful genotyping result,there were 163 samples of H1 a genotypes,2 samples of A genotypes,1 samples of B3,D9,G3 genotype,respectively. Of the 126 cases which Ig M negative or not detected,39 were PCR positive. Conclusion H1 a was the major epidemic genotype in Dongguan and various genotypes coexist together. D9 was first found in Guangdong. Serology and molecular biology techniques should be improved to improve the detection rate of measles pathogen surveillance.

关 键 词：麻疹病毒 血清学 分子生物学 基因型 

分 类 号：R511.1[医药卫生—内科学]