山东分离株鸡腺胃型传染性支气管炎的病理组织学和电镜观察  

Histopathology and Electron Microscopy Observation of Shandong Isolates of Glandular Stomach Type Avian Infectious Bronchitis Virus

作　　者：王玉东[1] 王永玲[1] 王君玮[1] 王晓燕[2] 高艳艳[1] 王树双[1] 谭金山[3] 牛钟相[4] 

机构地区：[1]中国动物卫生与流行病学中心,山东青岛266032 [2]中国农业大学国际合作与交流处,北京海淀100083 [3]青岛大学医学院,山东青岛266021 [4]山东农业大学动物科技学院,山东泰安271018

出　　处：《中国兽医杂志》2015年第10期24-27,I0002,共5页Chinese Journal of Veterinary Medicine

摘　　要：为探讨鸡腺胃型传染性支气管炎病毒（腺胃型IBV）引起鸡的病理组织变化和病毒超微结构特征,采用常规临床剖检、病理组织学显微观察技术及电子显微镜观察技术,对山东省3株病毒分离株（QX、DY、FC）引起的病理组织学变化和病毒的超微结构进行观察。结果表明,分离毒株可引起明显的腺胃组织炎症,主要表现腺胃黏膜内炎性细胞浸润,腺胃腺体上皮细胞增生,腺腔中有黏液、有多量坏死、脱落、崩解的腺细胞,以及单核细胞浸润,上皮细胞有多量空泡化等特点。在病理组织中观察到了大小为80-120m的病毒颗粒;通过鸡胚分离的病毒大小为80-150nm,该病毒略呈球形或梨形,有囊膜,囊膜表面覆有长12-25 nm的呈典型冠状的突起（团集的病毒则较少有冠状突起）,为冠状病毒。该结果为进一步研究和有效防治腺胃型传染性支气管炎提供了科学参考。In order to investigate histo-pathological changes of chickens induced by glandular stomach（GS）type avian infectious bronchitis virus and the ultra-structure of the virus,clinical dissection,histo-pathology and electron microscopic examination were conducted over the 3 Shandong isolates（QX,DY and FC） of stomach type avian infectious bronchitis virus and the ultrastructure of the virus.The results showed that the isolates induced significant glandular stomach tissue inflammation manifested mainly in inflammatory cell infiltration in proventriculus mucosa,glandular epithelial hyperplasia,mucus and abundant necrotic,exfoliated and disintegrated glandular cells in gland lumen,monocyte infiltration and abundant vacuolation in epithelial cells.Viral particles with 80-120 nm were observed in the pathological tissues.Virus isolated via chicken embryo was too 80-150 nm in size with spherical or pear-shaped form.The virus was enveloped with 12-25 typical coronoid projections（the clustered virus has few coronoid projections）,thus named as coronavirus.The study provided scientific reference for further studies and effective prevention and control of glandular stomach type avian infectious bronchitis.

关 键 词：鸡 传染性支气管炎 腺胃型 病毒 病理组织 电镜观察 

分 类 号：S852.659.6[农业科学—基础兽医学]