车前草多糖对雏鸡新城疫疫苗免疫效果的影响  

Effects of Plantain Polysaccharide on Newcastle disease vaccine in chicken

作　　者：陈家磊[1] 刘师旗 冯君[1] 郑业龙[1] 聂奎[1] 

机构地区：[1]西南大学动物科技学院,重庆北碚400715

出　　处：《中国兽医杂志》2015年第10期50-52,56,共4页Chinese Journal of Veterinary Medicine

基　　金：教育部国家级大学生创新创业训练计划(201210-635014);重庆市科委重点攻关项目(CSTC;2011AB2030)

摘　　要：为研究车前草多糖对雏鸡新城疫疫苗免疫效果的影响,将350只雏鸡随机分为7组,于8日龄、22日龄进行新城疫疫苗首免和二免,同时给予车前草多糖,并以黄芪多糖和铝胶盐佐剂作为对照,分别在首免后第7、14天,二免后第7、14、21天进行血清抗体效价和淋巴细胞增殖率的测定,于二免后第21天用新城疫病毒进行攻毒,连续观察10d内鸡的发病及死亡情况。结果显示,车前草多糖能显著提高雏鸡新城疫疫苗的免疫抗体效价、增强雏鸡的淋巴细胞增值率,与黄芪多糖的作用无显著性差异(P>0.05),但车前草多糖提高抗体效价作用较黄芪多糖、铝胶盐佐剂的维持时间长;在新城疫病毒攻毒后10 d,未免疫组雏鸡全部死亡,车前草多糖高、中剂量组的攻毒保护率为100%,车前草多糖低剂量和黄芪多糖的保护率为90%,而铝胶佐剂和ND疫苗免疫组的保护率仅为70%。试验结果表明,车前草多糖能提高新城疫疫苗的抗体效价水平和增强T淋巴细胞的增殖率。The effect of Plantain polysaccharide on Newcastle disease vaccine in chicken was evaluated.In our study,three hundred and fifty 8 day-old chickens were randomly divided into seven groups and each group was vaccinated with NDV at age of8 and 22 day old respectively.On days 7 and 14 after first vaccination and on days 7,14 and 21 after second vaccination,3 chicken from each group were bled for determination of serum ND-HI antibody titer and the proliferation of peripheral T lymphocyte.Ten chickens in each group were challenged by Newcastle disease virus on day 21 after second vaccination.The results showed that the ND-HI titer and the proliferation of peripheral T lymphocyte in Plantain polysaccharide＋NDV group were significantly higher than that in control group.Our data suggest that Plantain polysaccharid can significantly enhance the ND-HI titer when compared with Astragalus polysaccharide and Alhydrogel adjuvant.

关 键 词：车前草多糖 新城疫疫苗 T淋巴细胞 抗体 雏鸡 

分 类 号：S852.659.5[农业科学—基础兽医学]