8种生物农药对温郁金叶枯病病原菌的室内毒力测定  

Toxicity Measurement of Eight Biological Pesticides on Curvularia clavata from Curcuma Wen yujin in Laboratory

作　　者：陈旭玉[1,2] 钟锦玲 贺春萍[4] 黄娴 冯锦东[1] 甘炳春[1] 

机构地区：[1]中国医学科学院&北京协和医学院药用植物研究所海南分所(海南省南药资源保护与开发重点实验室),海南万宁571533 [2]中国医学科学院&北京协和医学院药用植物研究所,北京100193 [3]岭南师范学院,广东湛江524048 [4]中国热带农业科学院环境与植物保护研究所(农业部热带作物有害生物综合治理重点实验室),海口571101 [5]海南碧凯医药研究所,海口570216

出　　处：《中国农学通报》2015年第33期279-283,共5页Chinese Agricultural Science Bulletin

基　　金：国家科技重大专项子课题"中药材种子种苗和种植(养殖)标准平台"(2012ZX09304006);海南省重大专项子课题"特色南药;黎药中成药药材种植推广及产品的二次开发"(ZDZX2013008);国家基本药物所需中药原料资源调查和监测项目"国家基本药物所需中药材种子种苗繁育基地(海南)建设"[国中医药科技中药便函(2012)157;158号]

摘　　要：为了筛选出有效防治温郁金弯孢霉叶枯病的药剂,采用菌丝生长速率法测定8种生物农药对温郁金弯孢霉叶枯病病原菌(Curvularia clavata)的抑菌效果。8种生物农药对温郁金叶枯病病原菌抑菌效果好的为0.5%氨基寡糖素AS、3%中生菌WP、荧光假单胞杆菌WP和枯草芽孢杆菌WP。毒力最强为荧光假单胞杆菌WP,EC50值为0.93 mg/L;其次为哈茨木霉菌WP、枯草芽孢杆菌WP、0.5%氨基寡糖素AS和3%中生菌素WP,EC50分别为1.30、3.27、6.14、8.42 mg/L。荧光假单胞杆菌、0.5%氨基寡糖素AS、枯草芽孢杆菌和3%中生菌素WP均可有效抑制温郁金叶枯病病原菌的生长,可进一步用于田间试验。This study aims to select biological pesticide that can effectively control Curvularia clavata on Curcuma Wen yujin Chen et C.Ling. The antimicrobial effects of eight kinds of biological pesticide on C. clavata were tested by mycelium growth rate method. 0.5% amino-oligosaccharin AS, 3% Zhongsheng mycin WP, Pseudomonas fluorescens WP and Bacillus subtilis WP had better antimicrobial effects on C. clavata. In addition, the toxicity of P. fluorescens WP was the strongest, with the EC50 of 0.93 mg/L, followed byTrichoderma harzianum WP, B. subtilis WP, 0.5% amino-oligosaccharin AS and 3% Zhongsheng mycin WP with the ECho of 1.30, 3.27, 6.14, 8.42 mg/L, respectively. P. fluorescens WP, 0.5% amino-oligosaccharin AS, B. subtilis WP and 3% Zhongsheng mycin WP could effectively inhibit the growth of C. clavata and could be used in filed trials.

关 键 词：温郁金 弯孢霉叶枯病 病原菌 生物农药 毒力测定 

分 类 号：S482.2[农业科学—农药学]