下呼吸道分离的多重耐药铜绿假单胞菌的外排泵基因检测  

Detection of resistance genes associated with efflux pump for multidrugresistant Pseudomonas aeruginosa isolated from lower respiratory tract

作　　者：徐羽中[1] 何臣[1] 孙顺昌[1] 向开富[1] 袁利[1] 

机构地区：[1]南方医科大学附属深圳宝安人民医院,广东深圳518101

出　　处：《热带医学杂志》2015年第10期1332-1336,共5页Journal of Tropical Medicine

基　　金：深圳市宝安区科技基金(2014039)

摘　　要：目的检测下呼吸道分离的多重耐药铜绿假单胞菌的外排泵相关基因,探讨其耐药机制。方法收集40株从下呼吸道分离出的多重耐药铜绿假单胞菌,通过PCR扩增其外排泵相关的耐药基因(mexA、mexB、mexC、mexR、OprD、OprN),分析外排泵相关耐药基因与抗生素耐药性的关系。结果在40株下呼吸道分离的多重耐药铜绿假单胞菌中,检测到全部6种外排泵相关耐药基因,mexB基因携带率最高为100.0%,mexA基因携带率最低仅为7.5%,携带两种以上耐药基因的菌株占85%。其中头孢类药物和β-内酰胺酶类药物耐药和mexC基因的表达相关,亚胺培南类药物耐药和OprD基因有关。结论下呼吸道分离的多重耐药铜绿假单胞菌均携带外排泵相关基因,它可能是铜绿假单胞菌产生多重耐药的重要机制。Objective To detect resistance genes associated with efflux pump for multidrug-resistant pseudomonas isolated from lower respiratory tract,and explore its mechanism of multidrug-resistant of Pseudomonas aeruginosa. Methods Forty strains of multidrug-resistant Pseudomonas aeruginosa were isolated from lower respiratory tract. Resistance genes associated with efflux pump including mexA,mexB,mexC,mexR,OprD,and OprN were amplificated by polymerase chain reaction.Relationship of multidrug-resistance and resistance genes associated with efflux pump was analyzed. Results Six genes associated with efflux pump were all amplificated from these strains of multidrug-resistant Pseudomonas aeruginosa isolated from lower respiratory tract. mexB gene was amplified from 40 strains of Pseudomonas aeruginosa. mexA gene was amplified from 7.5% Pseudomonas aeruginosa strains. Eighty-five percent of the strains carried 2 genes associated with efflux pump.mexC gene was related to cephalosporins-resistance and beta-lactamase-resistance. OprD gene was related to imipenemresistance. Conclusion Genes associated with efflux pump can be found in multidrug-resistant Pseudomonas aeruginosa isolated from lower respiratory tract. Genes associated with efflux pump may be an important mechanism for multidrugresistant.

关 键 词：铜绿假单胞菌 耐药性 外排泵基因 

分 类 号：R378.99[医药卫生—病原生物学]