2011-2013年某医院血液标本检出病原菌的分布及耐药性分析  

Clinical distribution and drug-resistance characteristic of pathogens isolated from blood culture during 2011-2013

作　　者：陈超[1] 王永涛[1] 贾珉[1] 贾征夫[1] 苏斌涛[1] 

机构地区：[1]武汉市第一医院检验科,湖北武汉430022

出　　处：《热带医学杂志》2015年第10期1374-1376,1412,共4页Journal of Tropical Medicine

摘　　要：目的了解2011-2013年某医院血液标本中检出常见病原菌的分布及药物敏感情况。方法采用美国BD公司血培养仪和法国梅里埃公司血培养仪培养,VITEK-2 Compact全自动细菌鉴定仪进行鉴定及药敏试验。结果2011-2013年共分离出950株病原菌,以革兰阴性菌为主;大肠埃希菌检出率较高,3年分别占41.9%、28.6%、27.3%,其中产超广谱β-内酰胺酶（ESBLs）占43.8%~60.8%。2013年凝固酶阴性葡萄球菌占20.1%。2011-2013年耐甲氧西林金黄色葡萄球菌的检出率分别为13.3%、33.3%和43.2%,耐甲氧西林的凝固酶阴性葡萄球菌的检出率分别为50.0%、76.9%和86.1%。结论血液标本检出病原菌的分布及耐药有显著变化,临床应根据血液培养结果合理用药。Objective To understand the distribution and drug resistance of common pathogenic bacteria which cultured from blood specimens in the past 3 years. Methods Pathogens were cultured using BD blood culture systems and French Merieux blood culture system. VITEK-2 Compact automatic bacterial identification instrument was used for identification and drug sensitivity test of the isolated pathogens. Results In the past 3 years,there were 950 isolated pathogenic bacteria,mainly gram-negative bacteria. In the years 2011-2013,the culture rates of Escherichia coli were 41.9%,28.6%,and 27.3%,respectively. In which,the isolation rate of extended-spectrum β-lactamases（ESBLs） was 43.8%~60.8%. In 2013,the culture rate of coagulase negative staphylococci was 20.1%. From 2011 to 2013,the isolation rates of methicillin-resistant Staphylococcus aureus were 13.3%,33.3% and 43.2%,respectively,for methicillin-resistant coagulase-negative staphylococci were 50.0%,76.9% and 86.1%,respectively. Conclusion Distribution and antibiotic resistance of pathogenic bacteria cultured from blood specimens have a significant change in the past 3 years. Clinician should use the antibiotics reasonably according to the result of blood culture.

关 键 词：病原菌 药敏试验 耐药性 

分 类 号：R378[医药卫生—病原生物学]