小鼠子宫蜕膜基质细胞促进树突状细胞的增殖  

The mouse decidual stroma cells inducing the proliferation of dendritic cells in vitro

作　　者：李娜[1] 高美华[1] 

机构地区：[1]青岛大学医学院免疫学教研室,山东青岛266071

出　　处：《泰山医学院学报》2015年第5期481-485,共5页Journal of Taishan Medical College

基　　金：国家自然科学基金项目(30872322)

摘　　要：目的建立小鼠子宫蜕膜基质细胞(DSC)和小鼠骨髓来源的树突状细胞(DC)细胞共培养体系,探讨DSC对DC增殖的影响。方法应用白细胞介素-4(IL-4)和粒细胞-巨噬细胞集落刺激因子(GM-CSF)诱导小鼠骨髓细胞分化为DC,FACS检测细胞表面分子和细胞增殖,ELISA检测其分泌的细胞因子,构建DSC和DC共培养体系模型,动态观察DSC对DC增殖的影响。结果小鼠骨髓来源DC高表达CD11c,共刺激分子CD80和CD86,MHCⅡ类分子Ia和MHCⅠ类分子H-2Kb。经LPS刺激的DC分泌细胞因子IL-12(p70)、IL-6、TNF-α和IL-1β的水平明显上调(P<0.05),并能够促进抗原肽特异性T淋巴细胞增殖反应能力(P<0.05)。在DSC和DC共培养10天后观察到DSC明显促进DC增殖。结论小鼠子宫蜕膜基质细胞能够促进树突状细胞的增殖能力。Objective：To investigate the effects of decidual stroma cell（ DSC）on the proliferation of dendritic cells （ DC）by developing a co-culture system with the two cells. Methods：Cells isolated from the mice bone marrow were cul-tured with GM-CSF and IL-4 cytoKines,then cell surface marKers and cell proliferation were detected by FACS,and cyto-Kines were assayed by ELISA. Moreover,we developed a co-culture system with DSC and DC,and dynamically observed the effects of DSC on the proliferation of DC. Results：Bone marrow derived DC expressed high levels of CD11c,CD80, CD86,MHC-II,and MHC-I molecules. The secretion of IL-12（p70）,IL-6,TNF-αand IL-1 were increased significantly treated by LPS（P〈0. 05）,and the ability to stimulate antigen specific T cell proliferation was also enhanced（P〈0. 05）. In addition,the proliferation of DC was increased after we cocultrued DSC and DC for 10 days. Conclusion：The proliferation of DC increases when induced by the mice DSC in vitro.

关 键 词：蜕膜基质细胞 树突状细胞 共培养 细胞增殖 

分 类 号：R392[医药卫生—免疫学]